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@ARTICLE{Planert:285048,
author = {Planert, Henrike and Mittermaier, Franz Xaver and Grosser,
Sabine and Fidzinski, Pawel and Schneider, Ulf Christoph and
Radbruch, Helena and Onken, Julia and Holtkamp, Martin and
Schmitz, Dietmar and Alle, Henrik and Vida, Imre and Geiger,
Jörg Rolf Paul and Peng, Yangfan},
title = {{E}lectrophysiological classification of human layer 2-3
pyramidal neurons reveals subtype-specific synaptic
interactions.},
journal = {Nature neuroscience},
volume = {29},
number = {2},
issn = {1097-6256},
address = {New York, NY},
publisher = {Nature America},
reportid = {DZNE-2026-00172},
pages = {455 - 466},
year = {2026},
abstract = {Understanding the functional principles of the human brain
requires deep insight into its neuronal and network
physiology. In superficial layers of temporal cortex,
molecular and morphological subtypes of glutamatergic
excitatory pyramidal neurons have been described, but
subtyping based on electrophysiological parameters has not
been performed. The extent to which pyramidal neuron
subtypes contribute to the specialization of physiological
interactions by forming synaptic subnetworks remains
unclear. Here we performed whole-cell patch-clamp recordings
of more than 1,400 layer 2-3 (L2-3) pyramidal neurons and
1,400 identified monosynaptic connections in acute slices of
human temporal cortex. We extract principles of neuronal and
synaptic physiology along with anatomy and functional
synaptic connectivity. We also show robust classification of
pyramidal neurons into four electrophysiological subtypes,
corroborated by differences in morphology and decipher
subtype-specific synaptic interactions. Principles of
microcircuit organization are found to be conserved at the
individual level. Such a fine network structure suggests
that the functional diversity of pyramidal neurons
translates into differential computations within the L2-3
microcircuit of the human cortex.},
keywords = {Humans / Pyramidal Cells: physiology / Pyramidal Cells:
classification / Pyramidal Cells: cytology / Synapses:
physiology / Patch-Clamp Techniques / Temporal Lobe:
physiology / Temporal Lobe: cytology / Male / Female / Adult
/ Nerve Net: physiology / Middle Aged},
cin = {AG Schmitz},
ddc = {610},
cid = {I:(DE-2719)1810004},
pnm = {351 - Brain Function (POF4-351)},
pid = {G:(DE-HGF)POF4-351},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:41372679},
pmc = {pmc:PMC12880919},
doi = {10.1038/s41593-025-02134-7},
url = {https://pub.dzne.de/record/285048},
}