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000285359 1001_ $$0P:(DE-2719)9003500$$aHoenigsperger, Helene$$b0$$udzne
000285359 245__ $$aProtocol to differentially quantify spatially resolved viral protein-cellular protein interactions via proximity ligation assays.
000285359 260__ $$aCambridge, MA$$bCell Press$$c2026
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000285359 520__ $$aThe spatial organization of viral and cellular proteins shapes signal transduction. Here, we present a protocol to quantify differential protein-protein interactions by measuring their spatial association using proximity ligation assays (PLAs). We describe steps for seeding, transfection, proximity labeling, and confocal microscopy imaging and provide procedures for quantitative analysis. Our approach complements co-immunoprecipitation-based interactome data by enabling in situ quantification of differential binding between cellular and viral interaction partners. For complete details on the use and execution of this protocol, please refer to Klute et al.1.
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000285359 650_7 $$2Other$$aAntibody
000285359 650_7 $$2Other$$aImmunology
000285359 650_7 $$2Other$$aIn Situ Hybridization
000285359 650_7 $$2Other$$aMicrobiology
000285359 650_7 $$2Other$$aMolecular/Chemical Probes
000285359 7001_ $$0P:(DE-2719)9003510$$aKlute, Susanne$$b1$$udzne
000285359 7001_ $$0P:(DE-2719)9003514$$aEngels, Zoe$$b2$$udzne
000285359 7001_ $$aVolcic, Meta$$b3
000285359 7001_ $$0P:(DE-2719)9003481$$aSparrer, Konstantin Maria Johannes$$b4$$eLast author$$udzne
000285359 773__ $$0PERI:(DE-600)3053335-1$$a10.1016/j.xpro.2026.104361$$gVol. 7, no. 1, p. 104361 -$$n1$$p104361$$tSTAR Protocols$$v7$$x2666-1667$$y2026
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