Journal Article

DZNE-2026-00224

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Protocol to differentially quantify spatially resolved viral protein-cellular protein interactions via proximity ligation assays.

Hoenigsperger, H.Extern* ; Klute, S.Extern* ; Engels, Z.Extern* ; Volcic, M. ; Sparrer, K. M. J. (Last author)DZNE*

2026
Cell Press Cambridge, MA

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Please use a persistent id in citations: doi:10.1016/j.xpro.2026.104361

Abstract: The spatial organization of viral and cellular proteins shapes signal transduction. Here, we present a protocol to quantify differential protein-protein interactions by measuring their spatial association using proximity ligation assays (PLAs). We describe steps for seeding, transfection, proximity labeling, and confocal microscopy imaging and provide procedures for quantitative analysis. Our approach complements co-immunoprecipitation-based interactome data by enabling in situ quantification of differential binding between cellular and viral interaction partners. For complete details on the use and execution of this protocol, please refer to Klute et al.1.

Keyword(s): Antibody ; Immunology ; In Situ Hybridization ; Microbiology ; Molecular/Chemical Probes

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Contributing Institute(s):

1. Neurovirology and Neuroinflammation (AG Sparrer)

Research Program(s):

1. 351 - Brain Function (POF4-351) (POF4-351)

Appears in the scientific report 2026

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Database coverage:
; ; Article Processing Charges ; BIOSIS Previews ; Biological Abstracts ; Clarivate Analytics Master Journal List ; DOAJ Seal ; Emerging Sources Citation Index ; Fees ; SCOPUS ; Web of Science Core Collection

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