TY  - JOUR
AU  - Hoenigsperger, Helene
AU  - Klute, Susanne
AU  - Engels, Zoe
AU  - Volcic, Meta
AU  - Sparrer, Konstantin Maria Johannes
TI  - Protocol to differentially quantify spatially resolved viral protein-cellular protein interactions via proximity ligation assays.
JO  - STAR Protocols
VL  - 7
IS  - 1
SN  - 2666-1667
CY  - Cambridge, MA
PB  - Cell Press
M1  - DZNE-2026-00224
SP  - 104361
PY  - 2026
AB  - The spatial organization of viral and cellular proteins shapes signal transduction. Here, we present a protocol to quantify differential protein-protein interactions by measuring their spatial association using proximity ligation assays (PLAs). We describe steps for seeding, transfection, proximity labeling, and confocal microscopy imaging and provide procedures for quantitative analysis. Our approach complements co-immunoprecipitation-based interactome data by enabling in situ quantification of differential binding between cellular and viral interaction partners. For complete details on the use and execution of this protocol, please refer to Klute et al.1.
KW  - Antibody (Other)
KW  - Immunology (Other)
KW  - In Situ Hybridization (Other)
KW  - Microbiology (Other)
KW  - Molecular/Chemical Probes (Other)
LB  - PUB:(DE-HGF)16
C6  - pmid:41686640
C2  - pmc:PMC12915154
DO  - DOI:10.1016/j.xpro.2026.104361
UR  - https://pub.dzne.de/record/285359
ER  -