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@ARTICLE{Hoenigsperger:285359,
      author       = {Hoenigsperger, Helene and Klute, Susanne and Engels, Zoe
                      and Volcic, Meta and Sparrer, Konstantin Maria Johannes},
      title        = {{P}rotocol to differentially quantify spatially resolved
                      viral protein-cellular protein interactions via proximity
                      ligation assays.},
      journal      = {STAR Protocols},
      volume       = {7},
      number       = {1},
      issn         = {2666-1667},
      address      = {Cambridge, MA},
      publisher    = {Cell Press},
      reportid     = {DZNE-2026-00224},
      pages        = {104361},
      year         = {2026},
      abstract     = {The spatial organization of viral and cellular proteins
                      shapes signal transduction. Here, we present a protocol to
                      quantify differential protein-protein interactions by
                      measuring their spatial association using proximity ligation
                      assays (PLAs). We describe steps for seeding, transfection,
                      proximity labeling, and confocal microscopy imaging and
                      provide procedures for quantitative analysis. Our approach
                      complements co-immunoprecipitation-based interactome data by
                      enabling in situ quantification of differential binding
                      between cellular and viral interaction partners. For
                      complete details on the use and execution of this protocol,
                      please refer to Klute et al.1.},
      keywords     = {Antibody (Other) / Immunology (Other) / In Situ
                      Hybridization (Other) / Microbiology (Other) /
                      Molecular/Chemical Probes (Other)},
      cin          = {AG Sparrer},
      ddc          = {600},
      cid          = {I:(DE-2719)1910003},
      pnm          = {351 - Brain Function (POF4-351)},
      pid          = {G:(DE-HGF)POF4-351},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:41686640},
      pmc          = {pmc:PMC12915154},
      doi          = {10.1016/j.xpro.2026.104361},
      url          = {https://pub.dzne.de/record/285359},
}