guest ::
| Home > Publications Database > Software: CureDem_2026_reproducibility_scripts, v1 |
| Home > Publications Database > Software: CureDem_2026_reproducibility_scripts, v1 |
| Software | DZNE-2026-00487 |
; ;
2026
This record in other databases:
Please use a persistent id in citations: doi:10.5281/ZENODO.16894749
Abstract: Spintge, Mai, Carraro et al., 2026: Single-cell multi-omic profiling allows the dissection of peripheral immune phenotypes in Alzheimer’s Disease progression The role of the peripheral immune system in Alzheimer’s Disease (AD) remains insufficiently resolved, limiting the understanding of systemic disease effects and mechanisms. Here, we employed three high-resolution single-cell techniques, including flow cytometry, single-cell RNA- and ATAC-sequencing, to investigate peripheral immunity in AD dementia and earlier stages of the AD trajectory in over 100 patients. We identified reduced humoral immune responses in AD, characterized by a diminished B cell compartment displaying an impaired activation phenotype. Classical monocytes expanded in mild cognitive impairment and early AD dementia, acquiring a NF-kB/AP-1-mediated low-grade inflammation phenotype, validated in cerebrospinal fluid. We further identified nonlinear modulations of immune functionality and proportion stratifying disease progression to later-stage AD. Our findings link peripheral dysregulation in innate and adaptive immunity at cell frequency, transcriptional and epigenetic levels to the AD trajectory and provide insights into distinct phenotypes that define AD progression in contrast to healthy aging across cohorts. This repository contains the code and scripts compiled by Jannis Spintge, Karola Mai and Caterina Carraro to recreate the scRNA-seq, flow cytometry, and scATAC-seq figures from the manuscript. Data generated during this study are currently deposited at the Clinical Research Platform of the DZNE.
|
The record appears in these collections: |