Journal Article

DZNE-2020-04231

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png A genome wide association study links glutamate receptor pathway to sporadic Creutzfeldt-Jakob disease risk.

Sanchez-Juan, P. ; Bishop, M. T. ; Kovacs, G. G. ; Calero, M. ; Aulchenko, Y. S. ; Ladogana, A. ; Boyd, A. ; Lewis, V. ; Ponto, C.DZNE* ; Calero, O. ; Poleggi, A. ; Carracedo, Á. ; van der Lee, S. J. ; Ströbel, T. ; Rivadeneira, F. ; Hofman, A. ; Haïk, S. ; Combarros, O. ; Berciano, J. ; Uitterlinden, A. G. ; Collins, S. J. ; Budka, H. ; Brandel, J.-P. ; Laplanche, J. L. ; Pocchiari, M. ; Zerr, I.DZNE* ; Knight, R. S. G. ; Will, R. G. ; van Duijn, C. M.

2014
PLOS San Francisco, California, US

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Please use a persistent id in citations: doi:10.1371/journal.pone.0123654

Abstract: We performed a genome-wide association (GWA) study in 434 sporadic Creutzfeldt-Jakob disease (sCJD) patients and 1939 controls from the United Kingdom, Germany and The Netherlands. The findings were replicated in an independent sample of 1109 sCJD and 2264 controls provided by a multinational consortium. From the initial GWA analysis we selected 23 SNPs for further genotyping in 1109 sCJD cases from seven different countries. Five SNPs were significantly associated with sCJD after correction for multiple testing. Subsequently these five SNPs were genotyped in 2264 controls. The pooled analysis, including 1543 sCJD cases and 4203 controls, yielded two genome wide significant results: rs6107516 (p-value=7.62x10-9) a variant tagging the prion protein gene (PRNP); and rs6951643 (p-value=1.66x10-8) tagging the Glutamate Receptor Metabotropic 8 gene (GRM8). Next we analysed the data stratifying by country of origin combining samples from the pooled analysis with genotypes from the 1000 Genomes Project and imputed genotypes from the Rotterdam Study (Total n=12967). The meta-analysis of the results showed that rs6107516 (p-value=3.00x10-8) and rs6951643 (p-value=3.91x10-5) remained as the two most significantly associated SNPs. Rs6951643 is located in an intronic region of GRM8, a gene that was additionally tagged by a cluster of 12 SNPs within our top100 ranked results. GRM8 encodes for mGluR8, a protein which belongs to the metabotropic glutamate receptor family, recently shown to be involved in the transduction of cellular signals triggered by the prion protein. Pathway enrichment analyses performed with both Ingenuity Pathway Analysis and ALIGATOR postulates glutamate receptor signalling as one of the main pathways associated with sCJD. In summary, we have detected GRM8 as a novel, non-PRNP, genome-wide significant marker associated with heightened disease risk, providing additional evidence supporting a role of glutamate receptors in sCJD pathogenesis.

Keyword(s): Case-Control Studies (MeSH) ; Creutzfeldt-Jakob Syndrome: genetics (MeSH) ; Genome-Wide Association Study: methods (MeSH) ; Germany (MeSH) ; Humans (MeSH) ; Netherlands (MeSH) ; Polymorphism, Single Nucleotide (MeSH) ; Prion Proteins (MeSH) ; Prions: genetics (MeSH) ; Receptors, Metabotropic Glutamate: genetics (MeSH) ; Signal Transduction (MeSH) ; United Kingdom (MeSH) ; PRNP protein, human ; Prion Proteins ; Prions ; Receptors, Metabotropic Glutamate ; metabotropic glutamate receptor 8

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Contributing Institute(s):

1. Translational Studies and Biomarkers (AG Zerr)

Research Program(s):

1. 344 - Clinical and Health Care Research (POF3-344) (POF3-344)

Appears in the scientific report 2014

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