Journal Article

DZNE-2020-07011

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Disruption of Structural Disulfides of Coagulation FXIII-B Subunit; Functional Implications for a Rare Bleeding Disorder.

Singh, S. ; Akhter, M. S. ; Dodt, J. ; Sharma, A. ; Kaniyappan, S.DZNE* ; Yadegari, H. ; Ivaskevicius, V. ; Oldenburg, J. ; Biswas, A. (Corresponding author)

2019
Molecular Diversity Preservation International Basel

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Please use a persistent id in citations: doi:10.3390/ijms20081956

Abstract: Congenital FXIII deficiency is a rare bleeding disorder in which mutations are detected in F13A1 and F13B genes that express the two subunits of coagulation FXIII, the catalytic FXIII-A, and protective FXIII-B. Mutations in FXIII-B subunit are considerably rarer compared to FXIII-A. Three mutations in the F13B gene have been reported on its structural disulfide bonds. In the present study, we investigate the structural and functional importance of all 20 structural disulfide bonds in FXIII-B subunit. All disulfide bonds were ablated by individually mutating one of its contributory cysteine's, and these variants were transiently expressed in HEK293t cell lines. The expression products were studied for stability, secretion, the effect on oligomeric state, and on FXIII-A activation. The structural flexibility of these disulfide bonds was studied using classical MD simulation performed on a FXIII-B subunit monomer model. All 20 FXIII-B were found to be important for the secretion and stability of the protein since ablation of any of these led to a secretion deficit. However, the degree of effect that the disruption of disulfide bond had on the protein differed between individual disulfide bonds reflecting a functional hierarchy/diversity within these disulfide bonds.

Keyword(s): Blood Coagulation (MeSH) ; Blood Coagulation Disorders: blood (MeSH) ; Disulfides: chemistry (MeSH) ; Endoplasmic Reticulum: metabolism (MeSH) ; Factor XIII: chemistry (MeSH) ; Factor XIII: metabolism (MeSH) ; Humans (MeSH) ; Models, Molecular (MeSH) ; Protein Conformation (MeSH) ; Protein Multimerization (MeSH) ; Protein Subunits: chemistry (MeSH) ; Protein Subunits: metabolism (MeSH) ; Structure-Activity Relationship (MeSH) ; Disulfides ; Protein Subunits ; Factor XIII

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Contributing Institute(s):

1. Cell and Animal Models of Neurodegeneration (AG Mandelkow 2)

Research Program(s):

1. 342 - Disease Mechanisms and Model Systems (POF3-342) (POF3-342)

Appears in the scientific report 2019

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Database coverage:
; ; ; ; Clarivate Analytics Master Journal List ; Current Contents - Physical, Chemical and Earth Sciences ; DOAJ Seal ; Ebsco Academic Search ; IF >= 5 ; JCR ; SCOPUS ; Web of Science Core Collection

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