Journal Article

DZNE-2020-00374

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Functional implications of Cav 2.3 R-type voltage-gated calcium channels in the murine auditory system - novel vistas from brainstem-evoked response audiometry.

Lundt, A. ; Soós, J. ; Seidel, R. ; Henseler, C. ; Müller, R. ; Raj Ginde, V. ; Imran Arshaad, M. ; Ehninger, D.DZNE* ; Hescheler, J. ; Sachinidis, A. ; Broich, K. ; Wormuth, C. ; Papazoglou, A. ; Weiergräber, M. (Corresponding author)

2020
Wiley Oxford [u.a.]

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Please use a persistent id in citations: doi:10.1111/ejn.14591

Abstract: Voltage-gated Ca2+ channels (VGCCs) are considered to play a key role in auditory perception and information processing within the murine inner ear and brainstem. In the past, Cav 1.3 L-type VGCCs gathered most attention as their ablation causes congenital deafness. However, isolated patch-clamp investigation and localization studies repetitively suggested that Cav 2.3 R-type VGCCs are also expressed in the cochlea and further components of the ascending auditory tract, pointing to a potential functional role of Cav 2.3 in hearing physiology. Thus, we performed auditory profiling of Cav 2.3+/+ controls, heterozygous Cav 2.3+/- mice and Cav 2.3 null mutants (Cav 2.3-/- ) using brainstem-evoked response audiometry. Interestingly, click-evoked auditory brainstem responses (ABRs) revealed increased hearing thresholds in Cav 2.3+/- mice from both genders, whereas no alterations were observed in Cav 2.3-/- mice. Similar observations were made for tone burst-related ABRs in both genders. However, Cav 2.3 ablation seemed to prevent mutant mice from total hearing loss particularly in the higher frequency range (36-42 kHz). Amplitude growth function analysis revealed, i.a., significant reduction in ABR wave WI and WIII amplitude in mutant animals. In addition, alterations in WI -WIV interwave interval were observed in female Cav 2.3+/- mice whereas absolute latencies remained unchanged. In summary, our results demonstrate that Cav 2.3 VGCCs are mandatory for physiological auditory information processing in the ascending auditory tract.

Keyword(s): Acoustic Stimulation (MeSH) ; Animals (MeSH) ; Audiometry, Evoked Response (MeSH) ; Auditory Threshold (MeSH) ; Brain Stem (MeSH) ; Calcium Channels (MeSH) ; Calcium Channels, N-Type (MeSH) ; Evoked Potentials, Auditory, Brain Stem (MeSH) ; Female (MeSH) ; Male (MeSH) ; Mice (MeSH)

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Contributing Institute(s):

1. Translational Biogerontology (AG Ehninger)

Research Program(s):

1. 342 - Disease Mechanisms and Model Systems (POF3-342) (POF3-342)

Appears in the scientific report 2020

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Database coverage:
; ; ; BIOSIS Previews ; Biological Abstracts ; Clarivate Analytics Master Journal List ; Current Contents - Life Sciences ; DEAL Wiley ; Ebsco Academic Search ; Essential Science Indicators ; IF < 5 ; JCR ; Nationallizenz ; SCOPUS ; Web of Science Core Collection

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