Journal Article

DZNE-2021-00140

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Fine structure analysis of perineuronal nets in the ketamine model of schizophrenia.

Kaushik, R. (First author)DZNE* ; Lipachev, N.DZNE* ; Matuszko, G.DZNE* ; Kochneva, A. ; Dvoeglazova, A. ; Becker, A. ; Paveliev, M. ; Dityatev, A. (Last author)DZNE*

2021
Wiley Oxford [u.a.]

This record in other databases:    

Please use a persistent id in citations: doi:10.1111/ejn.14853

Abstract: Perineuronal nets (PNNs) represent a highly condensed specialized form of brain extracellular matrix (ECM) enwrapping mostly parvalbumin-positive interneurons in the brain in a mesh-like fashion. PNNs not only regulate the onset and completion of the critical period during postnatal brain development, control cell excitability, and synaptic transmission but are also implicated in several brain disorders including schizophrenia. Holes in the perineuronal nets, harboring the synaptic contacts, along with hole-surrounding ECM barrier can be viewed as PNN compartmentalization units that might determine the properties of synapses and heterosynaptic communication. In this study, we developed a novel open-source script for Fiji (ImageJ) to semi-automatically quantify structural alterations of PNNs such as the number of PNN units, area, mean intensity of PNN marker expression in 2D and 3D, shape parameters of PNN units in the ketamine-treated Sprague-Dawley rat model of schizophrenia using high-resolution confocal microscopic images. We discovered that the mean intensity of ECM within PNN units is inversely correlated with the area and the perimeter of the PNN holes. The intensity, size, and shape of PNN units proved to be three major principal factors to describe their variability. Ketamine-treated rats had more numerous but smaller and less circular PNN units than control rats. These parameters allowed to correctly classify individual PNNs as derived from control or ketamine-treated groups with ≈85% reliability. Thus, the proposed multidimensional analysis of PNN units provided a robust and comprehensive morphometric fingerprinting of fine ECM structure abnormalities in the experimental model of schizophrenia.

Keyword(s): Animals (MeSH) ; Extracellular Matrix (MeSH) ; Ketamine (MeSH) ; Rats (MeSH) ; Rats, Sprague-Dawley (MeSH) ; Reproducibility of Results (MeSH) ; Schizophrenia (MeSH) ; Wisteria floribunda agglutinin ; chondroitin sulfate proteoglycans ; extracellular matrix ; parvalbumin ; synapses

Note: ISSN 1460-9568 not unique: **3 hits**.

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Contributing Institute(s):

1. Molecular Neuroplasticity (AG Dityatev)

Research Program(s):

1. 342 - Disease Mechanisms and Model Systems (POF3-342) (POF3-342)
2. 351 - Brain Function (POF4-351) (POF4-351)

Appears in the scientific report 2021

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Database coverage:
; ; ; BIOSIS Previews ; Biological Abstracts ; Clarivate Analytics Master Journal List ; Current Contents - Life Sciences ; DEAL Wiley ; Ebsco Academic Search ; Essential Science Indicators ; IF < 5 ; JCR ; Nationallizenz ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection

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Linked articles:

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Dataset Dityatev, A.DZNE* ; Dityatev, A. (Other)DZNE*
Dataset: PNN unit analysis, v1
Mendeley (2022) [10.17632/cvf924fz3p.1]2022 BibTeX | EndNote: XML, Text | RIS

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