Journal Article

DZNE-2021-01188

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Systematically defining selective autophagy receptor-specific cargo using autophagosome content profiling.

Zellner, S. ; Schifferer, M.DZNE* ; Behrends, C. (Corresponding author)

2021
Elsevier New York, NY

This record in other databases:    

Please use a persistent id in citations: doi:10.1016/j.molcel.2021.01.009

Abstract: Autophagy deficiency in fed conditions leads to the formation of protein inclusions highlighting the contribution of this lysosomal delivery route to cellular proteostasis. Selective autophagy pathways exist that clear accumulated and aggregated ubiquitinated proteins. Receptors for this type of autophagy (aggrephagy) include p62, NBR1, TOLLIP, and OPTN, which possess LC3-interacting regions and ubiquitin-binding domains (UBDs), thus working as a bridge between LC3/GABARAP proteins and ubiquitinated substrates. However, the identity of aggrephagy substrates and the redundancy of aggrephagy and related UBD-containing receptors remains elusive. Here, we combined proximity labeling and organelle enrichment with quantitative proteomics to systematically map the autophagic degradome targeted by UBD-containing receptors under basal and proteostasis-challenging conditions in human cell lines. We identified various autophagy substrates, some of which were differentially engulfed by autophagosomal and endosomal membranes via p62 and TOLLIP, respectively. Overall, this resource will allow dissection of the proteostasis contribution of autophagy to numerous individual proteins.

Keyword(s): Autophagosomes: genetics (MeSH) ; Autophagosomes: metabolism (MeSH) ; Autophagy (MeSH) ; Autophagy-Related Proteins: genetics (MeSH) ; Autophagy-Related Proteins: metabolism (MeSH) ; HEK293 Cells (MeSH) ; HeLa Cells (MeSH) ; Humans (MeSH) ; Protein Interaction Maps (MeSH) ; Proteolysis (MeSH) ; Proteomics (MeSH) ; Proteostasis (MeSH) ; Ubiquitination (MeSH) ; APEX2 ; SQSTM1/p62 ; TOLLIP ; aggrephagy ; autophagosomes ; autophagy ; endosomal microautophagy ; proteostasis imbalance ; proximity labeling ; selective autophagy receptors ; Autophagy-Related Proteins

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Contributing Institute(s):

1. Neuronal Cell Biology (AG Misgeld)

Research Program(s):

1. 351 - Brain Function (POF4-351) (POF4-351)

Appears in the scientific report 2021

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Database coverage:
; BIOSIS Previews ; Biological Abstracts ; Clarivate Analytics Master Journal List ; Current Contents - Life Sciences ; Ebsco Academic Search ; Essential Science Indicators ; IF >= 15 ; JCR ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection

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Linked articles:

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Dataset Behrends, C. ; Zellner, S. (Other) ; Schifferer, M. (Other)DZNE*
Dataset: Systematically defining selective autophagy receptor-specific cargo using autophagosome content profiling
Mendeley (2021) [10.17632/mwsncvr8rv.1]2021 BibTeX | EndNote: XML, Text | RIS

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