Journal Article

DZNE-2022-00854

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Recruitment of release sites underlies chemical presynaptic potentiation at hippocampal mossy fiber boutons.

Orlando, M. ; Dvorzhak, A. ; Bruentgens, F. ; Maglione, M. ; Rost, B. R.DZNE* ; Sigrist, S. J.DZNE* ; Breustedt, J. ; Schmitz, D. (Last author)DZNE*

2021
PLoS Lawrence, KS

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Please use a persistent id in citations: doi:10.1371/journal.pbio.3001149

Abstract: Synaptic plasticity is a cellular model for learning and memory. However, the expression mechanisms underlying presynaptic forms of plasticity are not well understood. Here, we investigate functional and structural correlates of presynaptic potentiation at large hippocampal mossy fiber boutons induced by the adenylyl cyclase activator forskolin. We performed 2-photon imaging of the genetically encoded glutamate sensor iGluu that revealed an increase in the surface area used for glutamate release at potentiated terminals. Time-gated stimulated emission depletion microscopy revealed no change in the coupling distance between P/Q-type calcium channels and release sites mapped by Munc13-1 cluster position. Finally, by high-pressure freezing and transmission electron microscopy analysis, we found a fast remodeling of synaptic ultrastructure at potentiated boutons: Synaptic vesicles dispersed in the terminal and accumulated at the active zones, while active zone density and synaptic complexity increased. We suggest that these rapid and early structural rearrangements might enable long-term increase in synaptic strength.

Keyword(s): Animals (MeSH) ; Colforsin: pharmacology (MeSH) ; Glutamic Acid: metabolism (MeSH) ; Male (MeSH) ; Mice, Inbred C57BL (MeSH) ; Microscopy, Fluorescence, Multiphoton (MeSH) ; Mossy Fibers, Hippocampal: drug effects (MeSH) ; Mossy Fibers, Hippocampal: metabolism (MeSH) ; Mossy Fibers, Hippocampal: ultrastructure (MeSH) ; Neurotransmitter Agents: metabolism (MeSH) ; Presynaptic Terminals: drug effects (MeSH) ; Presynaptic Terminals: metabolism (MeSH) ; Synaptic Vesicles: drug effects (MeSH) ; Synaptic Vesicles: metabolism (MeSH) ; Neurotransmitter Agents ; Colforsin ; Glutamic Acid

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Contributing Institute(s):

1. Network Dysfunction (AG Schmitz)
2. Aging of Synapse Structure and Function (AG Sigrist)

Research Program(s):

1. 351 - Brain Function (POF4-351) (POF4-351)

Appears in the scientific report 2021

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Database coverage:
; ; ; ; Article Processing Charges ; BIOSIS Previews ; Biological Abstracts ; Clarivate Analytics Master Journal List ; Current Contents - Agriculture, Biology and Environmental Sciences ; Current Contents - Life Sciences ; DOAJ Seal ; Ebsco Academic Search ; Essential Science Indicators ; Fees ; IF >= 5 ; JCR ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection ; Zoological Record

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Linked articles:

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Dataset Orlando, M. ; Dvorzhak, A. ; Bruentgens, F. ; Maglione, M. ; Rost, B. R.DZNE* ; Sigrist, S. J.Extern* ; Breustedt, J. M. ; Schmitz, D.Extern*
Dataset: Recruitment of release sites underlies chemical presynaptic potentiation at hippocampal mossy fiber boutons
Zenodo (2021) [10.5281/zenodo.4498214]2021 BibTeX | EndNote: XML, Text | RIS

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