Journal Article

DZNE-2025-00674

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Structural basis for OAS2 regulation and its antiviral function.

Merold, V. ; Bekere, I. ; Kretschmer, S. ; Schnell, A. F. ; Kmiec, D. ; Sivarajan, R.Extern* ; Lammens, K. ; Liu, R. ; Mergner, J. ; Teppert, J. ; Hirschenberger, M.Extern* ; Henrici, A. ; Hammes, S. ; Buder, K. ; Weitz, M. ; Hackmann, K. ; Koenig, L. M. ; Pichlmair, A. ; Schwierz, N. ; Sparrer, K. M. J.DZNE* ; Lee-Kirsch, M. A. ; de Oliveira Mann, C. C.

2025
Cell Press [Cambridge, Mass.]

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Please use a persistent id in citations: doi:10.1016/j.molcel.2025.05.001

Abstract: Oligoadenylate synthetase (OAS) proteins are immune sensors for double-stranded RNA and are critical for restricting viruses. OAS2 comprises two OAS domains, only one of which can synthesize 2'-5'-oligoadenylates for RNase L activation. Existing structures of OAS1 provide a model for enzyme activation, but they do not explain how multiple OAS domains discriminate RNA length. Here, we discover that human OAS2 exists in an auto-inhibited state as a zinc-mediated dimer and present a mechanism for RNA length discrimination: the catalytically deficient domain acts as a molecular ruler that prevents autoreactivity to short RNAs. We demonstrate that dimerization and myristoylation localize OAS2 to Golgi membranes and that this is required for OAS2 activation and the restriction of viruses that exploit the endomembrane system for replication, e.g., coronaviruses. Finally, our results highlight the non-redundant role of OAS proteins and emphasize the clinical relevance of OAS2 by identifying a patient with a loss-of-function mutation associated with autoimmune disease.

Keyword(s): 2',5'-Oligoadenylate Synthetase: genetics (MeSH) ; 2',5'-Oligoadenylate Synthetase: chemistry (MeSH) ; 2',5'-Oligoadenylate Synthetase: metabolism (MeSH) ; Humans (MeSH) ; Golgi Apparatus: enzymology (MeSH) ; Golgi Apparatus: virology (MeSH) ; Protein Multimerization (MeSH) ; HEK293 Cells (MeSH) ; Virus Replication (MeSH) ; Mutation (MeSH) ; RNA, Double-Stranded: metabolism (MeSH) ; RNA, Double-Stranded: genetics (MeSH) ; HeLa Cells (MeSH) ; Endoribonucleases: metabolism (MeSH) ; Endoribonucleases: genetics (MeSH) ; Structure-Activity Relationship (MeSH) ; Adenine Nucleotides (MeSH) ; Oligoribonucleotides (MeSH) ; OAS2 ; RNA sensing ; RNase L ; innate immunity ; localization ; oligoadenylates ; structural biology ; virus restriction ; 2',5'-Oligoadenylate Synthetase ; OAS2 protein, human ; 2',5'-oligoadenylate ; RNA, Double-Stranded ; 2-5A-dependent ribonuclease ; Endoribonucleases ; Adenine Nucleotides ; Oligoribonucleotides

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Contributing Institute(s):

1. Neurovirology and Neuroinflammation (AG Sparrer)

Research Program(s):

1. 351 - Brain Function (POF4-351) (POF4-351)

Appears in the scientific report 2025

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Database coverage:
; ; ; BIOSIS Previews ; Biological Abstracts ; Clarivate Analytics Master Journal List ; Current Contents - Life Sciences ; Ebsco Academic Search ; Essential Science Indicators ; IF >= 15 ; JCR ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection

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