Journal Article

DZNE-2026-01093

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Improving optogenetic control over Gi/o-coupled receptor signaling by optimized GIRK channel expression.

Mücher, B. ; Garrido-Charles, A. ; Wietek, J. ; Köhler, I. ; Grömmke, M. ; Frischmuth, T. ; Cyganek, L. ; Rost, B. R.DZNE* ; Bruegmann, T. ; Schmitz, D.DZNE* ; Dalkara, D. ; Wahle, P. ; Siveke, I. ; Herlitze, S.

2026
Assoc. Washington, DC [u.a.]

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Please use a persistent id in citations: doi:10.1126/scisignal.adw4374

Abstract: Stimulation of G protein-coupled receptors (GPCRs) that couple to Gi/o leads to the activation of G protein-activated inward rectifier K+ (GIRK) channels and membrane hyperpolarization in excitable cells. GIRK channel availability can determine the success of optogenetic silencing strategies based on Gi/o-coupled GPCRs. To selectively enhance Gi/o-mediated GIRK currents, we created expression cassettes that coupled the activity of a GIRK subunit to the light-activated Gi/o-coupled GPCRs Opn4L (an opsin also known as melanopsin), longwave-sensitive opsin Opn1, parapinopsin, or Opn7b. We used the GIRK1 F137S mutant because of its fast activation, generation of large currents, and formation of homomeric channels, which reduced the size of the expression cassette. We demonstrated that light-mediated activation of GIRK1F137S through the opsins induced robust GIRK currents in HEK293 cells, cardiomyocytes, and cerebellar Purkinje neurons. Moreover, coupling GIRK1F137S activation to the promiscuous Opn4L changed the net effect of G protein signaling from Gq/11-mediated excitation to Gi/o-mediated inhibition in cerebellar Purkinje neurons and cardiomyocytes. Thus, our tools optimize the use of GIRK activity in the optogenetic control of the Gi/o pathway in excitable cells.

Keyword(s): Humans (MeSH) ; HEK293 Cells (MeSH) ; G Protein-Coupled Inwardly-Rectifying Potassium Channels: genetics (MeSH) ; G Protein-Coupled Inwardly-Rectifying Potassium Channels: metabolism (MeSH) ; Optogenetics: methods (MeSH) ; Animals (MeSH) ; Signal Transduction (MeSH) ; GTP-Binding Protein alpha Subunits, Gi-Go: metabolism (MeSH) ; GTP-Binding Protein alpha Subunits, Gi-Go: genetics (MeSH) ; Rod Opsins: metabolism (MeSH) ; Rod Opsins: genetics (MeSH) ; Myocytes, Cardiac: metabolism (MeSH) ; Melanopsin (MeSH) ; Receptors, G-Protein-Coupled: metabolism (MeSH) ; Receptors, G-Protein-Coupled: genetics (MeSH) ; Mice (MeSH) ; G Protein-Coupled Inwardly-Rectifying Potassium Channels ; GTP-Binding Protein alpha Subunits, Gi-Go ; Rod Opsins ; Melanopsin ; Receptors, G-Protein-Coupled

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Contributing Institute(s):

1. Network Dysfunction (AG Schmitz)

Research Program(s):

1. 351 - Brain Function (POF4-351) (POF4-351)

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Database coverage:
; BIOSIS Previews ; Biological Abstracts ; Clarivate Analytics Master Journal List ; Essential Science Indicators ; IF >= 5 ; JCR ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection

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