Journal Article

DZNE-2020-02729

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Three-dimensional imaging of the unsectioned adult spinal cord to assess axon regeneration and glial responses after injury.

Ertürk, A. ; Mauch, C. P. ; Hellal, F.DZNE* ; Förstner, F. ; Keck, T. ; Becker, K. ; Jährling, N. ; Steffens, H. ; Richter, M. ; Hübener, M. ; Kramer, E. ; Kirchhoff, F. ; Dodt, H. U. ; Bradke, F. (Last author)DZNE*

2012
Nature America Inc. New York, NY

This record in other databases:    

Please use a persistent id in citations: doi:10.1038/nm.2600

Abstract: Studying regeneration in the central nervous system (CNS) is hampered by current histological and imaging techniques because they provide only partial information about axonal and glial reactions. Here we developed a tetrahydrofuran-based clearing procedure that renders fixed and unsectioned adult CNS tissue transparent and fully penetrable for optical imaging. In large spinal cord segments, we imaged fluorescently labeled cells by 'ultramicroscopy' and two-photon microscopy without the need for histological sectioning. We found that more than a year after injury growth-competent axons regenerated abundantly through the injury site. A few growth-incompetent axons could also regenerate when they bypassed the lesion. Moreover, we accurately determined quantitative changes of glial cells after spinal cord injury. Thus, clearing CNS tissue enables an unambiguous evaluation of axon regeneration and glial reactions. Our clearing procedure also renders other organs transparent, which makes this approach useful for a large number of preclinical paradigms.

Keyword(s): Animals (MeSH) ; Axons: physiology (MeSH) ; Axons: ultrastructure (MeSH) ; Furans: chemistry (MeSH) ; Imaging, Three-Dimensional: methods (MeSH) ; Mice (MeSH) ; Microglia: physiology (MeSH) ; Microglia: ultrastructure (MeSH) ; Microscopy, Confocal: methods (MeSH) ; Spinal Cord Injuries: physiopathology (MeSH) ; Spinal Cord Regeneration (MeSH) ; Furans ; tetrahydrofuran

---

Contributing Institute(s):

1. Axon Growth and Regeneration (AG Bradke)

Research Program(s):

1. 341 - Molecular Signaling (POF3-341) (POF3-341)

Appears in the scientific report 2011

---

Database coverage:
; BIOSIS Previews ; Clarivate Analytics Master Journal List ; Current Contents - Clinical Medicine ; Current Contents - Life Sciences ; Ebsco Academic Search ; IF >= 30 ; JCR ; NCBI Molecular Biology Database ; Nationallizenz ; SCOPUS ; Science Citation Index ; Science Citation Index Expanded ; Web of Science Core Collection

---