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| Home > Publications Database > Cell-type-specific profiling of brain mitochondria reveals functional and molecular diversity. |
| Home > Publications Database > Cell-type-specific profiling of brain mitochondria reveals functional and molecular diversity. |
| Journal Article | DZNE-2020-07867 |
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2019
Nature Publ. Group62203
London
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Please use a persistent id in citations: doi:10.1038/s41593-019-0479-z
Abstract: Mitochondria vary in morphology and function in different tissues; however, little is known about their molecular diversity among cell types. Here we engineered MitoTag mice, which express a Cre recombinase-dependent green fluorescent protein targeted to the outer mitochondrial membrane, and developed an isolation approach to profile tagged mitochondria from defined cell types. We determined the mitochondrial proteome of the three major cerebellar cell types (Purkinje cells, granule cells and astrocytes) and identified hundreds of mitochondrial proteins that are differentially regulated. Thus, we provide markers of cell-type-specific mitochondria for the healthy and diseased mouse and human central nervous systems, including in amyotrophic lateral sclerosis and Alzheimer's disease. Based on proteomic predictions, we demonstrate that astrocytic mitochondria metabolize long-chain fatty acids more efficiently than neuronal mitochondria. We also characterize cell-type differences in mitochondrial calcium buffering via the mitochondrial calcium uniporter (Mcu) and identify regulator of microtubule dynamics protein 3 (Rmdn3) as a determinant of endoplasmic reticulum-mitochondria proximity in Purkinje cells. Our approach enables exploring mitochondrial diversity in many in vivo contexts.
Keyword(s): Alzheimer Disease: metabolism (MeSH) ; Alzheimer Disease: pathology (MeSH) ; Amyotrophic Lateral Sclerosis: metabolism (MeSH) ; Amyotrophic Lateral Sclerosis: pathology (MeSH) ; Animals (MeSH) ; Astrocytes: metabolism (MeSH) ; Brain: cytology (MeSH) ; Calcium Signaling: genetics (MeSH) ; Calcium Signaling: physiology (MeSH) ; Cells, Cultured (MeSH) ; Cerebellum: cytology (MeSH) ; Fatty Acids: metabolism (MeSH) ; Humans (MeSH) ; Mice (MeSH) ; Mice, Transgenic (MeSH) ; Mitochondria: metabolism (MeSH) ; Mitochondrial Membranes: metabolism (MeSH) ; Neurons: metabolism (MeSH) ; Proteomics (MeSH) ; Purkinje Cells: metabolism (MeSH) ; Fatty Acids
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