Abstract/Journal Article

DZNE-2020-00719

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png P.199: Determination of the sample-storagedependent stability on the RT-QuIC signal

Cramm, M. (First author)DZNE* ; Schmitz, M.DZNE* ; Zerr, I. (Last author)DZNE*

2014

Abstract: Background. Creutzfeldt-Jakob disease (CJD) is caused byscrapie prion protein (PrPSc), the pathogenic form of cellularprion protein (PrPC), which is able to convert PrPC into PrPSc.1About 85% of the CJD cases appear sporadic (sCJD).2 Real-timequaking-induced conversion (RT-QuIC) uses the self-propagating properties of PrPSc to amplify it up to a detectable limit, bythe use of recombinant prion protein (rPrP) as a substrate andintermittent automated shaking. Fluorescence detection of aggregated PrPSc monitors amplification in real-time.3 Recent studiesshowed the potential of RT-QuIC to detect PrPSc in CSF of CJDpatients.4, 5, 6,7 In the present study we analyzed the influence ofsample storage conditions (StC) on the stability of the RT-QuICsignal

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Contributing Institute(s):

1. Translational Studies and Biomarker (AG Zerr)

Research Program(s):

1. 344 - Clinical and Health Care Research (POF3-344) (POF3-344)

Appears in the scientific report 2014

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Database coverage:
; ; ; ; Article Processing Charges ; BIOSIS Previews ; Biological Abstracts ; Clarivate Analytics Master Journal List ; DOAJ Seal ; Essential Science Indicators ; Fees ; IF < 5 ; JCR ; NCBI Molecular Biology Database ; PubMed Central ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection

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