Journal Article (Editorial)

DZNE-2021-01003

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Screening Arrays of Laminin Peptides on Modified Cellulose for Promotion of Adhesion of Primary Endothelial and Neural Precursor Cells

Wetzel, R.Extern* ; Hauser, S. ; Lin, W. ; Berg, P. ; Werner, C. ; Pietzsch, J. ; Kempermann, G.DZNE* ; Zhang, Y.

2021
Wiley-VCH Weinheim

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Please use a persistent id in citations: doi:10.1002/adbi.201900303

Abstract: Neural precursor cells (NPC) are primary cells intensively used in the context of research on adult neurogenesis and modeling of neuronal development in health and diseased states. Substrates that can facilitate NPC adhesion will be very useful for culturing these cells. Due to the presence of laminin in basal lamina as well as their involvement in differentiation, migration, and adhesion of many types of cells, surfaces modified with laminin-derived peptides are focused upon and compared with the widely used fibronectin-derived Arg-Gly-Asp (RGD) peptides. An array of 46 peptides is synthesized on cellulose paper (SPOT) to identify laminin-derived peptides that promote short-term adhesion of murine NPC and human primary endothelial cells. Various previously reported peptide sequences are re-evaluated in this work. Initial adhesion experiments show NPC preferred several laminin-derived peptides by up to 5-time higher cell numbers, compared to the well-known promiscuous integrin binding RGD peptide. Importantly, screening of cell adhesion has revealed a synergetic effect of filamentous matrix, peptide sequence, surface property, ligand density, and the dynamic process of NPC adhesion.

Note: (CC BY)

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Contributing Institute(s):

1. Adult Neurogenesis (AG Kempermann 1 ; AG Kempermann)

Research Program(s):

1. 352 - Disease Mechanisms (POF4-352) (POF4-352)

Appears in the scientific report 2021

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Database coverage:
; ; ; BIOSIS Previews ; Biological Abstracts ; Clarivate Analytics Master Journal List ; Current Contents - Engineering, Computing and Technology ; DEAL Wiley ; Essential Science Indicators ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection

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