guest ::
| Home > Publications Database > Unbiased proteomic profiling reveals the IP3R modulator AHCYL1/IRBIT as a novel interactor of microtubule-associated protein tau. |
| Home > Publications Database > Unbiased proteomic profiling reveals the IP3R modulator AHCYL1/IRBIT as a novel interactor of microtubule-associated protein tau. |
| Journal Article | DZNE-2022-00468 |
; ; ; ; ; ; ; ; ;
2022
American Soc. for Biochemistry and Molecular Biology
Bethesda, MD.
This record in other databases: 
Please use a persistent id in citations: doi:10.1016/j.jbc.2022.101774
Abstract: Microtubule-associated protein tau is a naturally unfolded protein that can modulate a vast array of physiological processes through direct or indirect binding with molecular partners. Aberrant tau homeostasis has been implicated in the pathogenesis of several neurodegenerative disorders, including Alzheimer's disease. In this study, we performed an unbiased high-content protein profiling assay by incubating recombinant human tau on microarrays containing thousands of human polypeptides. Among the putative tau-binding partners, we identify SAH hydrolase-like protein 1/inositol 1,4,5-trisphosphate receptor (IP3R)-binding protein (AHCYL1/IRBIT), a member of the SAH hydrolase family and a previously described modulator of IP3R activity. Using coimmunoprecipitation assays, we show that endogenous as well as overexpressed tau can physically interact with AHCYL1/IRBIT in brain tissues and cultured cells. Proximity ligation assay experiments demonstrate that tau overexpression may modify the close localization of AHCYL1/IRBIT to IP3R at the endoplasmic reticulum. Together, our experimental evidence indicates that tau interacts with AHCYL1/IRBIT and potentially modulates AHCYL1/IRBIT function.
Keyword(s): Endoplasmic Reticulum: genetics (MeSH) ; Endoplasmic Reticulum: metabolism (MeSH) ; Gene Expression (MeSH) ; Humans (MeSH) ; Lectins, C-Type: genetics (MeSH) ; Lectins, C-Type: metabolism (MeSH) ; Membrane Proteins: genetics (MeSH) ; Membrane Proteins: metabolism (MeSH) ; Protein Binding (MeSH) ; Proteomics (MeSH) ; tau Proteins: genetics (MeSH) ; tau Proteins: metabolism (MeSH) ; Alzheimer’s disease ; SAH hydrolase–like protein 1 (AHCYL1/IRBIT) ; autophagy ; human protein microarray ; microtubule-associated protein tau
; 
; 
; BIOSIS Previews ; Biological Abstracts ; Clarivate Analytics Master Journal List ; Current Contents - Life Sciences ; Ebsco Academic Search ; Essential Science Indicators ; IF < 5 ; JCR ; PubMed Central ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection
PDF
PDF (PDFA)