Journal Article

DZNE-2022-00485

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Dynamical component exchange in a model phase separating system: an NMR-based approach.

Pantoja Rivillas, C. (First author)DZNE* ; Zweckstetter, M.DZNE* ; Rezaei-Ghaleh, N.

2022
RSC Publ. Cambridge

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Please use a persistent id in citations: doi:10.1039/D2CP00042C

Abstract: Biomolecular phase separation plays a key role in the spatial organization of cellular activities. Dynamic formation and rapid component exchange between phase separated cellular bodies and their environment are crucial for their function. Here, we employ a well-established phase separating model system, namely, a triethylamine (TEA)-water mixture, and develop an NMR approach to detect the exchange of scaffolding TEA molecules between separate phases and determine the underlying exchange rate. We further demonstrate how the advantageous NMR properties of fluorine nuclei provide access to otherwise inaccessible exchange processes of a client molecule. The developed NMR-based approach allows quantitative monitoring of the effect of regulatory factors on component exchange and facilitates 'exchange'-based screening and optimization of small molecules against druggable biomolecular targets located inside condensed phases.

Keyword(s): Fluorine (MeSH) ; Humans (MeSH) ; Magnetic Resonance Imaging (MeSH) ; Magnetic Resonance Spectroscopy (MeSH) ; Models, Biological (MeSH) ; Nuclear Magnetic Resonance, Biomolecular (MeSH) ; Fluorine

Note: (CC BY-NC)

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Contributing Institute(s):

1. Structural Biology in Dementia (AG Zweckstetter)

Research Program(s):

1. 352 - Disease Mechanisms (POF4-352) (POF4-352)

Appears in the scientific report 2022

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Database coverage:
; ; ; Clarivate Analytics Master Journal List ; Current Contents - Physical, Chemical and Earth Sciences ; Essential Science Indicators ; IF < 5 ; JCR ; National-Konsortium ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection

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