Journal Article

DZNE-2023-00489

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Centrosomal microtubule nucleation regulates radial migration of projection neurons independently of polarization in the developing brain.

Vinopal, S. (First author)DZNE* ; Dupraz, S.DZNE* ; Alfadil, E.DZNE* ; Pietralla, T.DZNE* ; Bendre, S.DZNE* ; Stiess, M.DZNE* ; Falk, S. ; Camargo Ortega, G. ; Maghelli, N. ; Tolić, I. M. ; Smejkal, J. ; Götz, M. ; Bradke, F. (Last author)DZNE*

2023
Elsevier New York, NY

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Please use a persistent id in citations: doi:10.1016/j.neuron.2023.01.020

Abstract: Cortical projection neurons polarize and form an axon while migrating radially. Even though these dynamic processes are closely interwoven, they are regulated separately-the neurons terminate their migration when reaching their destination, the cortical plate, but continue to grow their axons. Here, we show that in rodents, the centrosome distinguishes these processes. Newly developed molecular tools modulating centrosomal microtubule nucleation combined with in vivo imaging uncovered that dysregulation of centrosomal microtubule nucleation abrogated radial migration without affecting axon formation. Tightly regulated centrosomal microtubule nucleation was required for periodic formation of the cytoplasmic dilation at the leading process, which is essential for radial migration. The microtubule nucleating factor γ-tubulin decreased at neuronal centrosomes during the migratory phase. As distinct microtubule networks drive neuronal polarization and radial migration, this provides insight into how neuronal migratory defects occur without largely affecting axonal tracts in human developmental cortical dysgeneses, caused by mutations in γ-tubulin.

Keyword(s): Humans (MeSH) ; Tubulin: metabolism (MeSH) ; Neurons: physiology (MeSH) ; Axons: metabolism (MeSH) ; Microtubules: metabolism (MeSH) ; Centrosome (MeSH) ; Brain: metabolism (MeSH) ; axon formation ; centrosome ; microtubule ; neuronal polarity ; radial migration ; Tubulin

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Contributing Institute(s):

1. Axon Growth and Regeneration (AG Bradke)

Research Program(s):

1. 351 - Brain Function (POF4-351) (POF4-351)

Appears in the scientific report 2023

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Database coverage:
; ; ; BIOSIS Previews ; Biological Abstracts ; Clarivate Analytics Master Journal List ; Current Contents - Life Sciences ; Ebsco Academic Search ; Essential Science Indicators ; IF >= 15 ; JCR ; Nationallizenz ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection

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Linked articles:

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Software Schölling, M.DZNE* ; Vinopal, S.
Software: Python scripts related a publication 'Centrosomal Microtubule Nucleation Regulates Radial Migration of Projection Neurons Independently of Polarization in the Developing Brain' (v1.0)
Zenodo (2023) [10.5281/ZENODO.7554028]2023 BibTeX | EndNote: XML, Text | RIS

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