Journal Article

DZNE-2023-00746

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Impaired ATF3 signaling involves SNAP25 in SOD1 mutant ALS patients.

Yazar, V. (First author)DZNE* ; Kühlwein, J.Extern* ; Knehr, A. ; Grozdanov, V.Extern* ; Ekici, A. B. ; Ludolph, A. C.DZNE* ; Danzer, K. M. (Last author)DZNE*

2023
Macmillan Publishers Limited, part of Springer Nature [London]

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Please use a persistent id in citations: doi:10.1038/s41598-023-38684-8

Abstract: Epigenetic remodeling is emerging as a critical process for several neurodegenerative diseases, including amyotrophic lateral sclerosis (ALS). Genetics alone fails to explain the etiology of ALS, the investigation of the epigenome might therefore provide novel insights into the molecular mechanisms of the disease. In this study, we interrogated the epigenetic landscape in peripheral blood mononuclear cells (PBMCs) of familial ALS (fALS) patients with either chromosome 9 open reading frame 72 (C9orf72) or superoxide dismutase 1 (SOD1) mutation and aimed to identify key epigenetic footprints of the disease. To this end, we used an integrative approach that combines chromatin immunoprecipitation targeting H3K27me3 (ChIP-Seq) with the matching gene expression data to gain new insights into the likely impact of blood-specific chromatin remodeling on ALS-related molecular mechanisms. We demonstrated that one of the hub molecules that modulates changes in PBMC transcriptome in SOD1-mutant ALS patients is ATF3, which has been previously reported in an SOD1G93A mouse model. We also identified potential suppression of SNAP25, with impaired ATF3 signaling in SOD1-mutant ALS blood. Together, our study shed light on the mechanistic underpinnings of SOD1 mutations in ALS.

Keyword(s): Animals (MeSH) ; Mice (MeSH) ; Amyotrophic Lateral Sclerosis: genetics (MeSH) ; Amyotrophic Lateral Sclerosis: metabolism (MeSH) ; Leukocytes, Mononuclear: metabolism (MeSH) ; Mice, Transgenic (MeSH) ; Mutation (MeSH) ; Superoxide Dismutase: genetics (MeSH) ; Superoxide Dismutase: metabolism (MeSH) ; Superoxide Dismutase-1: genetics (MeSH) ; Superoxide Dismutase ; Superoxide Dismutase-1 ; SNAP25 protein, human ; SOD1 protein, human

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Contributing Institute(s):

1. Mechanisms of Propagation (AG Danzer)
2. Clinical Study Center Ulm (Clinical Study Center Ulm)

Research Program(s):

1. 352 - Disease Mechanisms (POF4-352) (POF4-352)
2. 353 - Clinical and Health Care Research (POF4-353) (POF4-353)

Appears in the scientific report 2023

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