Journal Article (Review Article)

DZNE-2024-00220

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Whole blood stimulation as a tool for studying the human immune system.

Mueller, S. (First author)DZNE* ; Kroeger, C. (First author)DZNE* ; Schultze, J. L.DZNE* ; Aschenbrenner, A. C. (Last author)DZNE*

2024
Wiley-VCH Weinheim

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Please use a persistent id in citations: doi:10.1002/eji.202350519

Abstract: The human immune system is best accessible via tissues and organs not requiring major surgical intervention, such as blood. In many circumstances, circulating immune cells correlate with an individual's health state and give insight into physiological and pathophysiological processes. Stimulating whole blood ex vivo is a powerful tool to investigate immune responses. In the context of clinical research, the applications of whole blood stimulation include host immunity, disease characterization, diagnosis, treatment, and drug development. Here, we summarize different setups and readouts of whole blood assays and discuss applications for preclinical research and clinical practice. Finally, we propose combining whole blood stimulation with high-throughput technologies, such as single-cell RNA-sequencing, to comprehensively analyze the human immune system for the identification of biomarkers, therapeutic interventions as well as companion diagnostics.

Keyword(s): Blood (MeSH) ; Humans (MeSH) ; Immune System (MeSH) ; Whole blood ⋅ Ex vivo stimulation ⋅ Functional immune assay ⋅ Precision medicine ; Whole blood ⋅ Ex vivo stimulation ⋅ Functional immune assay ⋅ Precision medicine ; Whole blood ⋅ Ex vivo stimulation ⋅ Functional immune assay ⋅ Precision medicine

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Contributing Institute(s):

1. Aging and Immunity (AG Aschenbrenner)
2. Clinical Single Cell Omics (CSCO) / Systems Medicine (AG Schultze)
3. Platform for Single Cell Genomics and Epigenomics (PRECISE)

Research Program(s):

1. 354 - Disease Prevention and Healthy Aging (POF4-354) (POF4-354)

Experiment(s):

1. Platform for Single Cell Genomics and Epigenomics at DZNE University of Bonn

Appears in the scientific report 2024

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Database coverage:
; ; ; BIOSIS Previews ; Clarivate Analytics Master Journal List ; Current Contents - Life Sciences ; IF >= 5 ; JCR ; Nationallizenz ; SCOPUS ; Web of Science Core Collection

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