Journal Article

DZNE-2025-00728

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Effect of RNA on the supramolecular architecture of α-synuclein fibrils.

Intze, A. ; Temperini, M. E. ; Rupert, J.DZNE* ; Polito, R. ; Veber, A. ; Puskar, L. ; Schade, U. ; Ortolani, M. ; Zacco, E. ; Tartaglia, G. G. ; Giliberti, V.

2025
Cell Press Cambridge, Mass.

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Please use a persistent id in citations: doi:10.1016/j.bpj.2025.04.031

Abstract: Structural changes associated with protein aggregation are challenging to study, requiring the combination of experimental techniques providing insights at the molecular level across diverse scales, ranging from nanometers to microns. Understanding these changes is even more complex when aggregation occurs in the presence of molecular cofactors such as nucleic acids and when the resulting aggregates are highly polymorphic. Infrared (IR) spectroscopy is a powerful tool for studying protein aggregates since it combines the label-free sensitivity to the cross-β architecture, an inherent feature of protein supramolecular aggregates, with the possibility to reach nanoscale sensitivity by leveraging atomic force microscopy (AFM)-assisted detection. Here, we present a combined approach that detects IR spectral markers of aggregation using various IR spectroscopy techniques, covering micro-to-nanoscale ranges, to study the effect of RNA on the supramolecular architecture of α-synuclein amyloid aggregates. We show a clear impact of RNA consistent with enhanced intermolecular forces, likely via a stronger hydrogen-bonded network stabilizing the cross-β architecture. AFM-assisted IR spectroscopy was crucial to assess that the more ordered the aggregates are, the stronger the structural impact of RNA. In addition, an RNA-induced reduction of the degree of polymorphism within the aggregate population is obtained.

Keyword(s): alpha-Synuclein: chemistry (MeSH) ; alpha-Synuclein: metabolism (MeSH) ; RNA: chemistry (MeSH) ; RNA: metabolism (MeSH) ; Protein Aggregates (MeSH) ; Amyloid: chemistry (MeSH) ; Microscopy, Atomic Force (MeSH) ; Spectrophotometry, Infrared (MeSH) ; Humans (MeSH) ; alpha-Synuclein ; RNA ; Protein Aggregates ; Amyloid

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Contributing Institute(s):

1. Molecular Neuroscience (AG Milovanovic (Bonn))

Research Program(s):

1. 351 - Brain Function (POF4-351) (POF4-351)

Appears in the scientific report 2025

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Database coverage:
; ; ; BIOSIS Previews ; Biological Abstracts ; Clarivate Analytics Master Journal List ; Current Contents - Life Sciences ; Essential Science Indicators ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection

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