Dataset

DZNE-2026-00518

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Dataset: Data from: A human iPSC model of tauopathies engineered for 4R tau isoform expression endogenously develops late-stage neuronal tau pathology

Dannert, A. ; Schulz, N. ; Klimmt, J. ; Knez, L. ; Groschup, B. ; Gonçalves, C. ; Carraro, C.Extern* ; Schifferer, M.DZNE* ; Brendel, M.DZNE* ; Paquet, D.

2026
Dryad

This record in other databases:  

Please use a persistent id in citations: doi:10.5061/DRYAD.8CZ8W9H6G  doi:10.5061/dryad.8cz8w9h6g

Abstract: Tauopathies, such as Alzheimer’s disease and Frontotemporal Dementia, are common neurodegenerative diseases characterized by misfolding, hyperphosphorylation, and aggregation of Tau. Molecular mechanisms underlying Tauopathies are still poorly understood, in part due to a lack of human models endogenously developing major disease hallmarks. Adult Tau isoform expression contributes to Tau pathogenesis but is challenging to replicate in human stem-cell-derived systems, which impedes the formation of late-stage disease phenotypes and, hence, the research on underlying mechanisms and drug development. Here, we show that induction of adult human brain-like 4R Tau isoform expression enables endogenous formation of late-stage Tauopathy hallmarks in iPSC-derived neurons engineered to contain synergistic Tau mutations. Neurons accumulated seeding-competent, hyper-phosphorylated, fibrillar Tau in tangle-like structures. Furthermore, exclusive expression of mutant 4R in the absence of the 3R Tau isoform disproportionately intensified pathology, resulting in highly abundant Tau misfolding and aggregation. Finally, we provide proof-of-principle that our model can be translationally applied both to test chemical disease modulators and evaluate a human Tau PET tracer. Collectively, our model enables novel investigations on endogenous mechanisms of human Tauopathy formation, suggesting a central role of 4R Tau isoform expression for pathogenesis in human neurons. Moreover, it may also serve as a platform supporting urgently needed development of disease-modifying drugs.

Keyword(s): FOS: Biological sciences ; CRISPR ; Induced pluripotent stem cells ; Tau protein ; Dementia

---

Contributing Institute(s):

1. Neuronal Cell Biology (AG Misgeld)
2. Molecular Neurodegeneration (AG Haass)

Research Program(s):

1. 351 - Brain Function (POF4-351) (POF4-351)
2. 352 - Disease Mechanisms (POF4-352) (POF4-352)

Appears in the scientific report 2026

---

---

Linked articles:

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Journal Article Dannert, A. ; Schulz, N. ; Klimmt, J. ; Knez, L. ; Groschup, B. ; Gonçalves, C. C. ; Carraro, C.Extern* ; Schifferer, M.DZNE* ; Brendel, M.DZNE* ; Paquet, D.
A human iPSC model of tauopathies engineered for 4R tau isoform expression endogenously develops late-stage neuronal tau pathology.
Science translational medicine 18(844), eadu9845 (2026) [10.1126/scitranslmed.adu9845]2026   Files BibTeX | EndNote: XML, Text | RIS

---