Journal Article (Review Article)

DZNE-2026-00645

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png A Systematic Comparison of Alpha-Synuclein Seed Amplification Assays for Increasing Reproducibility.

Amaral-do-Nascimento, M. ; Santos, D. F. ; Vieira, T. C. R. G. ; Outeiro, T. F. (Last author)DZNE*

2026
Wiley Chichester [u.a.]

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Please use a persistent id in citations: doi:10.1002/acn3.70384

Abstract: Seed amplification assays (SAAs) enable ultrasensitive detection of misfolded α-synuclein across biofluids and tissues. Yet, heterogeneity in protocols limits cross-study comparability and clinical translation. Here, we review α-synuclein SAA methods and their performance across various biological matrices. We catalog key variables (e.g., human recombinant α-synuclein variants, buffers, salts, beads, shaking, incubation temperature) and outline pre-analytical factors that shape outcomes. We propose a prioritized framework of key analytical and pre-analytical variables most likely to influence assay reproducibility and clinical interpretation. We also outline minimal reporting criteria and practical quality-control checkpoints, and recommend multicenter interlaboratory proficiency testing as essential steps to accelerate the robust clinical and research implementation of α-synuclein SAAs.

Keyword(s): Humans (MeSH) ; alpha-Synuclein: analysis (MeSH) ; alpha-Synuclein: metabolism (MeSH) ; Reproducibility of Results (MeSH) ; Synucleinopathies: diagnosis (MeSH) ; RT‐QuIC ; alpha‐synuclein ; biomarker ; seed amplification assay ; synucleinopathies ; alpha-Synuclein

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Contributing Institute(s):

1. Epigenetics and Systems Medicine in Neurodegenerative Diseases (AG Fischer)

Research Program(s):

1. 352 - Disease Mechanisms (POF4-352) (POF4-352)

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Database coverage:
; ; Article Processing Charges ; Clarivate Analytics Master Journal List ; DEAL Wiley ; DOAJ Seal ; Ebsco Academic Search ; Essential Science Indicators ; Fees ; IF >= 5 ; JCR ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection

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