Journal Article

DZNE-2026-00710

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Inhibition of AXL receptor tyrosine kinase increases osteoblast function and bone mass.

Ahmad, M. ; Kölbl, C. ; Jan, I. ; Dorn, A.-K. ; Özkan, B. ; Olde Heuvel, F. ; Yilmaz, D. ; Wagner, A. ; Vujic Spasic, M. ; Krüger, B. T. ; Lee, S. ; Roselli, F.DZNE* ; Ignatius, A. ; Tuckermann, J.

2026
Nature Publ. Group [Erscheinungsort nicht ermittelbar]

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Please use a persistent id in citations: doi:10.1038/s41413-026-00554-0

Abstract: Osteoporosis, a prevalent age-related disease, is characterized by impaired bone formation and an increased risk of fractures. Current anabolic treatments primarily rely on biologics, which are costly and require inconvenient administration. Identifying regulators of osteoblastogenesis that are amenable to small-molecule targeting is essential for developing more accessible therapies. Through an unbiased kinome-wide RNAi screen in primary murine calvarial osteoblasts, we identified the AXL receptor tyrosine kinase (Axl) as a negative regulator of osteoblast differentiation. Axl is most highly expressed in undifferentiated and early differentiated osteoblasts, with a rapid decline in expression during osteoblast maturation. siRNA-mediated knockdown of Axl or pharmacological inhibition with the small molecule BGB324 significantly enhanced osteoblast differentiation and mineralization in vitro. In mice, BGB324 treatment significantly increased bone mass by promoting bone formation. Mechanistically, Axl knockdown or inhibition upregulated interferon-stimulated gene 15 (Isg15), while Isg15 knockdown impaired osteoblast differentiation and enhanced Erk phosphorylation, leading to increased expression of osteoblast-specific genes. Consistently, double knockdown experiments demonstrated that simultaneous loss of Axl with either Isg15 or Mapk1, but not other interferon-related genes, reversed the Axl knockdown-induced increase in osteoblast differentiation, reinforcing their mechanistic involvement. Collectively, our study identifies Axl as a promising therapeutic target for osteoporosis and other bone-related disorders.

Keyword(s): Osteoblasts: drug effects (MeSH) ; Osteoblasts: metabolism (MeSH) ; Osteoblasts: cytology (MeSH) ; Osteoblasts: enzymology (MeSH) ; Animals (MeSH) ; Receptor Protein-Tyrosine Kinases: antagonists & inhibitors (MeSH) ; Receptor Protein-Tyrosine Kinases: metabolism (MeSH) ; Receptor Protein-Tyrosine Kinases: genetics (MeSH) ; Axl Receptor Tyrosine Kinase (MeSH) ; Proto-Oncogene Proteins: antagonists & inhibitors (MeSH) ; Proto-Oncogene Proteins: metabolism (MeSH) ; Proto-Oncogene Proteins: genetics (MeSH) ; Cell Differentiation: drug effects (MeSH) ; Triazoles: pharmacology (MeSH) ; Osteogenesis: drug effects (MeSH) ; Benzocycloheptenes: pharmacology (MeSH) ; Mice (MeSH) ; Bone and Bones: drug effects (MeSH) ; Bone Density: drug effects (MeSH) ; Mice, Inbred C57BL (MeSH) ; Gene Knockdown Techniques (MeSH) ; Receptor Protein-Tyrosine Kinases ; Axl Receptor Tyrosine Kinase ; Proto-Oncogene Proteins ; AXL receptor tyrosine kinase, mouse ; Triazoles ; bemcentinib ; Benzocycloheptenes

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Contributing Institute(s):

1. Metabolic Changes in Neurodegeneration (AG Roselli)

Research Program(s):

1. 352 - Disease Mechanisms (POF4-352) (POF4-352)

Appears in the scientific report 2026

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Database coverage:
; ; ; ; Article Processing Charges ; Clarivate Analytics Master Journal List ; DOAJ Seal ; Essential Science Indicators ; Fees ; IF >= 10 ; JCR ; PubMed Central ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection

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