Dataset

DZNE-2026-01087

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Dataset: Bulk-RNA of MACS isolated Microglia from a Parkinson Mouse Model brain from young and old mice and Parkinson and Control, v1

Danzer, K. M.DZNE* ; Lee, J.DZNE*

2026
Zenodo

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Please use a persistent id in citations: doi:10.5281/zenodo.20412962

Abstract: Bulk RNA-seq dataset of microglia isolated from a transgenic Parkinson's disease mouse model (Camk2a-tTA; V1S/SV2 α-synuclein). Microglia were enriched from adult brain by enzymatic dissociation (Miltenyi Adult Brain Dissociation Kit), Anti-O4 oligodendrocyte depletion, and CD11b positive selection (~2 M cells per pooled sample). Total RNA (Zymo Quick-RNA Microprep) was used to prepare strand-specific libraries (QuantaBio sparQ RNA-Seq HMR), sequenced on a NovaSeq X (PE150, ~166 M reads/sample). Reads were trimmed (fastp v0.23.4) and aligned to mm39 extended with the Camk2a-tTA and V1S/SV2 transgenes (STAR v2.7.3a, GeneCounts).This deposit contains gene-level count data as a SummarizedExperiment with corresponding sample metadata.

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Contributing Institute(s):

1. Mechanisms of Propagation (AG Danzer)

Research Program(s):

1. 352 - Disease Mechanisms (POF4-352) (POF4-352)

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