Journal Article

DZNE-2020-04197

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Higher-order architecture of rhodopsin in intact photoreceptors and its implication for phototransduction kinetics.

Gunkel, M. ; Schöneberg, J. ; Alkhaldi, W.DZNE* ; Irsen, S. ; Noé, F. ; Kaupp, U. B. (Corresponding author) ; Alamoudi, A. (Last author)DZNE*

2015
Cell Press Cambridge, Mass.

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Please use a persistent id in citations: doi:10.1016/j.str.2015.01.015

Abstract: The visual pigment rhodopsin belongs to the family of G protein-coupled receptors that can form higher oligomers. It is controversial whether rhodopsin forms oligomers and whether oligomers are functionally relevant. Here, we study rhodopsin organization in cryosections of dark-adapted mouse rod photoreceptors by cryoelectron tomography. We identify four hierarchical levels of organization. Rhodopsin forms dimers; at least ten dimers form a row. Rows form pairs (tracks) that are aligned parallel to the disk incisures. Particle-based simulation shows that the combination of tracks with fast precomplex formation, i.e. rapid association and dissociation between inactive rhodopsin and the G protein transducin, leads to kinetic trapping: rhodopsin first activates transducin from its own track, whereas recruitment of transducin from other tracks proceeds more slowly. The trap mechanism could produce uniform single-photon responses independent of rhodopsin lifetime. In general, tracks might provide a platform that coordinates the spatiotemporal interaction of signaling molecules.

Keyword(s): Animals (MeSH) ; Kinetics (MeSH) ; Mice (MeSH) ; Mice, Inbred C57BL (MeSH) ; Photoreceptor Cells: metabolism (MeSH) ; Photoreceptor Cells: ultrastructure (MeSH) ; Protein Binding (MeSH) ; Protein Multimerization (MeSH) ; Rhodopsin: chemistry (MeSH) ; Rhodopsin: metabolism (MeSH) ; Transducin: metabolism (MeSH) ; Vision, Ocular (MeSH) ; Rhodopsin ; Transducin

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Contributing Institute(s):

1. Cryo-electron microscopy and tomography (AG Alamoudi)

Research Program(s):

1. 341 - Molecular Signaling (POF3-341) (POF3-341)

Appears in the scientific report 2015

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Database coverage:
; BIOSIS Previews ; Clarivate Analytics Master Journal List ; Current Contents - Life Sciences ; Ebsco Academic Search ; NCBI Molecular Biology Database ; Nationallizenz ; SCOPUS ; Science Citation Index ; Science Citation Index Expanded ; Web of Science Core Collection

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