ATUM-FIB microscopy for targeting and multiscale imaging of rare events in mouse cortex.

Kislinger, Georg; Kerschensteiner, Martin; Schifferer, Martina; Misgeld, Thomas; Gnägi, Helmut; Simons, Mikael

doi:10.1016/j.xpro.2020.100232

Journal Article

DZNE-2022-00935

ATUM-FIB microscopy for targeting and multiscale imaging of rare events in mouse cortex.

Kislinger, G. (First author)DZNE* ; Gnägi, H. ; Kerschensteiner, M. ; Simons, M.DZNE* ; Misgeld, T.DZNE* ; Schifferer, M. (Last author)DZNE*

2020
Elsevier Amsterdam

STAR Protocols 1(3), 100232 (2020) [10.1016/j.xpro.2020.100232]

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Abstract: Here, we describe a detailed workflow for ATUM-FIB microscopy, a hybrid method that combines serial-sectioning scanning electron microscopy (SEM) with focused ion beam SEM (FIB-SEM). This detailed protocol is optimized for mouse cortex samples. The main processing steps include the generation of semi-thick sections from sequentially cured resin blocks using a heated microtomy approach. We demonstrate the different imaging modalities, including serial light and electron microscopy for target recognition and FIB-SEM for isotropic imaging of regions of interest. For complete details on the use and execution of this protocol, please refer to Kislinger et al. (2020).

Keyword(s): Animals (MeSH) ; Cerebral Cortex: diagnostic imaging (MeSH) ; Cerebral Cortex: metabolism (MeSH) ; Image Processing, Computer-Assisted: methods (MeSH) ; Imaging, Three-Dimensional: methods (MeSH) ; Mice (MeSH) ; Microscopy, Electron, Scanning: methods (MeSH) ; Microtomy: instrumentation (MeSH) ; Microtomy: methods (MeSH) ; Workflow (MeSH) ; Microscopy ; Model organisms ; Neuroscience