Dataset

DZNE-2025-00805

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Dataset: Proteome analysis of amniotic fluid from fetuses with myelomeningocele from singleton pregnancies

Müller, S. A.DZNE* ; Lichtenthaler, S.DZNE*

2025
PRoteomics IDEntifications Database

Abstract: Despite numerous studies on fetal therapy for myelomeningoceles (MMC), the pathophysiology of this malformation remains poorly understood. This study aimed to analyze the biochemical profile and proteome of amniotic fluid (AF) supernatants from MMC fetuses to explore the prenatal pathophysiology. Proteome analysis was conducted in 18 MMC and 18 healthy singleton fetuses, as well as in 5 dichorionic pregnancies with MMC fetuses and their healthy co-twins. ELISA tests were used to validate proteome results. Biochemical analysis revealed anal incontinence in 37% of MMC cases (p<0.0001), while controls had a normal profile. Proteomics identified 2453 quantified proteins, with 39 significantly up-regulated and 10 down-regulated in MMC. Up-regulated proteins included ectodomains of CHL1, APLP1, SEZ6, SEZ6L, which are generated by the Alzheimer’s disease-linked protease BACE1. Some proteins varied with disease and gestational age, e.g., CNTN1, NEO1, and DRAXIN. COL11A2 and EFNB1 decreased in MMC, rising in controls. Contrary to the in-utero inflammation or meconium neurotoxicity hypothesis, our results suggest a CSF leak in AF. Abundance of brain and spinal cord proteins may aid diagnosis, characterizing cases and informing prognosis for couples.

---

Contributing Institute(s):

1. Neuroproteomics (AG Lichtenthaler)

Research Program(s):

1. 352 - Disease Mechanisms (POF4-352) (POF4-352)

Appears in the scientific report 2025

---

---

Linked articles:

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Journal Article Guilbaud, L. ; Roger, K. ; Schmidt, A.DZNE* ; Chhuon, C. ; Breimann, S.DZNE* ; Lipecka, J. ; Dreux, S. ; Müller, S. A.DZNE* ; Zérah, M. ; Larghero, J. ; Jouannic, J.-M. ; Lichtenthaler, S. F.DZNE* ; Guerrera, I. C.
Molecular insights into myelomeningocele via proteomic analysis of amniotic fluid.
Journal of proteomics 313, 105372 (2025) [10.1016/j.jprot.2024.105372]2025   Files BibTeX | EndNote: XML, Text | RIS

---