Journal Article

DZNE-2020-07247

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Tagger-A Swiss army knife for multiomics to dissect cell type-specific mechanisms of gene expression in mice.

Kaczmarczyk, L. (First author)DZNE* ; Bansal, V. ; Rajput, A. ; Rahman, R.-U. ; Krzyżak, W. ; Degen, J. ; Poll, S.DZNE* ; Fuhrmann, M.DZNE* ; Bonn, S.DZNE* ; Jackson, W. S. (Last author)DZNE*

2019
PLoS Lawrence, KS

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Please use a persistent id in citations: doi:10.1371/journal.pbio.3000374

Abstract: A deep understanding of how regulation of the multiple levels of gene expression in mammalian tissues give rise to complex phenotypes has been impeded by cellular diversity. A handful of techniques were developed to tag-select nucleic acids of interest in specific cell types, thereby enabling their capture. We expanded this strategy by developing the Tagger knock-in mouse line bearing a quad-cistronic transgene combining enrichment tools for nuclei, nascent RNA, translating mRNA, and mature microRNA (miRNA). We demonstrate that Tagger can capture the desired nucleic acids, enabling multiple omics approaches to be applied to specific cell types in vivo using a single transgenic mouse line.

Keyword(s): Animals (MeSH) ; Cloning, Molecular: methods (MeSH) ; Gene Expression: genetics (MeSH) ; Gene Expression Profiling: methods (MeSH) ; Gene Expression Regulation: genetics (MeSH) ; Gene Knock-In Techniques (MeSH) ; Genomics: methods (MeSH) ; Mice (MeSH) ; Mice, Inbred C57BL (MeSH) ; Mice, Transgenic: genetics (MeSH) ; MicroRNAs: genetics (MeSH) ; Nucleic Acids: isolation & purification (MeSH) ; Proteomics: methods (MeSH) ; RNA, Messenger: genetics (MeSH) ; Transcriptome: genetics (MeSH) ; Transgenes: genetics (MeSH) ; Whole Genome Sequencing: methods (MeSH)

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Contributing Institute(s):

1. Selective Vulnerability of Neurodegenerative Diseases (AG Jackson)
2. Neuroimmunology and Imaging (AG Fuhrmann)
3. Computational analysis of biological networks (AG Bonn 1)

Research Program(s):

1. 342 - Disease Mechanisms and Model Systems (POF3-342) (POF3-342)

Appears in the scientific report 2019

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Database coverage:
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