Journal Article

DZNE-2021-00163

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Local Efficacy of Glutamate Uptake Decreases with Synapse Size.

Herde, M. K. ; Bohmbach, K. ; Domingos, C. ; Vana, N. ; Komorowska-Müller, J. A. ; Passlick, S. ; Schwarz, I. ; Jackson, C. J. ; Dietrich, D. ; Schwarz, M. K. ; Henneberger, C. (Last author)DZNE*

2020
Elsevier [New York, NY]

This record in other databases:    

Please use a persistent id in citations: doi:10.1016/j.celrep.2020.108182

Abstract: Synaptically released glutamate is largely cleared by glutamate transporters localized on perisynaptic astrocyte processes. Therefore, the substantial variability of astrocyte coverage of individual hippocampal synapses implies that the efficacy of local glutamate uptake and thus the spatial fidelity of synaptic transmission is synapse dependent. By visualization of sub-diffraction-limit perisynaptic astrocytic processes and adjacent postsynaptic spines, we show that, relative to their size, small spines display a stronger coverage by astroglial transporters than bigger neighboring spines. Similarly, glutamate transients evoked by synaptic stimulation are more sensitive to pharmacological inhibition of glutamate uptake at smaller spines, whose high-affinity N-methyl-D-aspartate receptors (NMDARs) are better shielded from remotely released glutamate. At small spines, glutamate-induced and NMDAR-dependent Ca2+ entry is also more strongly increased by uptake inhibition. These findings indicate that spine size inversely correlates with the efficacy of local glutamate uptake and thereby likely determines the probability of synaptic crosstalk.

Keyword(s): Amino Acid Transport System X-AG: metabolism (MeSH) ; Animals (MeSH) ; Astrocytes: metabolism (MeSH) ; Calcium: metabolism (MeSH) ; Cell Size (MeSH) ; Dendritic Spines: metabolism (MeSH) ; Female (MeSH) ; Glutamic Acid: metabolism (MeSH) ; Male (MeSH) ; Mice (MeSH) ; Receptors, N-Methyl-D-Aspartate: metabolism (MeSH) ; Synapses: metabolism (MeSH) ; astrocyte morphology ; astrocytes ; expansion microscopy ; glutamate uptake ; hippocampus ; multiphoton imaging ; spine morphology

Note: ISSN 2211-1247 not unique: **3 hits**.

---

Contributing Institute(s):

1. U Preclinical Researchers - Bonn (U Preclinical Researchers - Bonn)

Research Program(s):

1. 342 - Disease Mechanisms and Model Systems (POF3-342) (POF3-342)

Appears in the scientific report 2020

---

Database coverage:
; ; ; ; Article Processing Charges ; BIOSIS Previews ; Biological Abstracts ; Clarivate Analytics Master Journal List ; DOAJ Seal ; Essential Science Indicators ; Fees ; IF >= 5 ; JCR ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection

---