Journal Article

DZNE-2022-01732

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Microtubule retrograde flow retains neuronal polarization in a fluctuating state.

Schelski, M. (First author)DZNE* ; Bradke, F. (Last author)DZNE*

2022
Assoc. Washington, DC [u.a.]

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Please use a persistent id in citations: doi:10.1126/sciadv.abo2336

Abstract: In developing vertebrate neurons, a neurite is formed by more than a hundred microtubules. While individual microtubules are dynamic, the microtubule array has been regarded as stationary. Using live-cell imaging of neurons in culture or in brain slices, combined with photoconversion techniques and pharmacological manipulations, we uncovered that the microtubule array flows retrogradely within neurites to the soma. This flow drives cycles of microtubule density, a hallmark of the fluctuating state before axon formation, thereby inhibiting neurite growth. The motor protein dynein fuels this process. Shortly after axon formation, microtubule retrograde flow slows down in the axon, reducing microtubule density cycles and enabling axon extension. Thus, keeping neurites short is an active process. Microtubule retrograde flow is a previously unknown type of cytoskeletal dynamics, which changes the hitherto axon-centric view of neuronal polarization.

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Contributing Institute(s):

1. Axon Growth and Regeneration (AG Bradke)

Research Program(s):

1. 351 - Brain Function (POF4-351) (POF4-351)

Appears in the scientific report 2022

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Linked articles:

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Software Bradke, F.DZNE* ; Schelski, M.DZNE*
Software: Microtubule retrograde flow retains neuronal polarization in a fluctuating state (v1)
Zenodo (2022) [10.5281/ZENODO.6990849]2022 BibTeX | EndNote: XML, Text | RIS

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Dataset Bradke, F. (First author)DZNE* ; Schelski, M. (Last author)DZNE*
Dataset: Microtubule retrograde flow retains neuronal polarization in a fluctuating state
Dryad (2022) [10.5061/dryad.2fqz612s8]2022 BibTeX | EndNote: XML, Text | RIS

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