Journal Article

DZNE-2023-00066

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Fast Motions Dominate Dynamics of Intrinsically Disordered Tau Protein at High Temperatures.

Abyzov, A.Extern* ; Mandelkow, E.DZNE* ; Zweckstetter, M.DZNE* ; Rezaei-Ghaleh, N. (Corresponding author)Extern*

2023
Wiley-VCH Weinheim

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Please use a persistent id in citations: doi:10.1002/chem.202203493

Abstract: Reorientational dynamics of intrinsically disordered proteins (IDPs) contain multiple motions often clustered around three motional modes: ultrafast librational motions of amide groups, fast local backbone conformational fluctuations and slow chain segmental motions. This dynamic picture is mainly based on 15 N NMR relaxation studies of IDPs at relatively low temperatures where the amide-water proton exchange rates are sufficiently small. Less is known, however, about the dynamics of IDPs at more physiological temperatures. Here, we investigate protein dynamics in a 441-residue long IDP, tau protein, in the temperature range from 0-25 °C, using 15 N NMR relaxation rates and spectral density analysis. While at these temperatures relaxation rates are still better described in terms of amide group librational motions, local backbone dynamics and chain segmental motions, the temperature-dependent trend of spectral densities suggests that the timescales of fast backbone conformational fluctuations and slower chain segmental motions might become inseparable at higher temperatures. Our data demonstrate the remarkable dynamic plasticity of this prototypical IDP and highlight the need for dynamic studies of IDPs at multiple temperatures.

Keyword(s): Temperature (MeSH) ; Protein Conformation (MeSH) ; tau Proteins (MeSH) ; Magnetic Resonance Spectroscopy (MeSH) ; Intrinsically Disordered Proteins: chemistry (MeSH) ; Amides (MeSH) ; tau Proteins ; Intrinsically disordered protein ; NMR relaxation ; protein dynamics ; spectral density analysis ; tau protein ; Intrinsically Disordered Proteins ; Amides

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Contributing Institute(s):

1. Structural Principles of Neurodegeneration (AG Mandelkow 1)
2. Structural Biology in Dementia (AG Zweckstetter)

Research Program(s):

1. 352 - Disease Mechanisms (POF4-352) (POF4-352)

Appears in the scientific report 2023

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Database coverage:
; ; ; Chemical Reactions ; Clarivate Analytics Master Journal List ; Current Contents - Physical, Chemical and Earth Sciences ; DEAL Wiley ; Ebsco Academic Search ; Essential Science Indicators ; IF < 5 ; Index Chemicus ; JCR ; Nationallizenz ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection

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