Journal Article

DZNE-2021-00213

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png An optimized quantitative proteomics method establishes the cell type-resolved mouse brain secretome.

Tüshaus, J. (First author)DZNE* ; Müller, S. A.DZNE* ; Kataka, E. S. ; Zaucha, J. ; Sebastian Monasor, L.DZNE* ; Su, M.DZNE* ; Güner, G.DZNE* ; Jocher, G.DZNE* ; Tahirovic, S.DZNE* ; Frishman, D. ; Simons, M.DZNE* ; Lichtenthaler, S. (Last author)DZNE*

2020
Wiley Hoboken, NJ [u.a.]

This record in other databases:    

Please use a persistent id in citations: doi:10.15252/embj.2020105693

Abstract: To understand how cells communicate in the nervous system, it is essential to define their secretome, which is challenging for primary cells because of large cell numbers being required. Here, we miniaturized secretome analysis by developing the 'high-performance secretome protein enrichment with click sugars' (hiSPECS) method. To demonstrate its broad utility, hiSPECS was used to identify the secretory response of brain slices upon LPS-induced neuroinflammation and to establish the cell type-resolved mouse brain secretome resource using primary astrocytes, microglia, neurons, and oligodendrocytes. This resource allowed mapping the cellular origin of CSF proteins and revealed that an unexpectedly high number of secreted proteins in vitro and in vivo are proteolytically cleaved membrane protein ectodomains. Two examples are neuronally secreted ADAM22 and CD200, which we identified as substrates of the Alzheimer-linked protease BACE1. hiSPECS and the brain secretome resource can be widely exploited to systematically study protein secretion and brain function and to identify cell type-specific biomarkers for CNS diseases.

Keyword(s): ADAM Proteins: cerebrospinal fluid (MeSH) ; ADAM Proteins: metabolism (MeSH) ; Amyloid Precursor Protein Secretases: antagonists & inhibitors (MeSH) ; Amyloid Precursor Protein Secretases: cerebrospinal fluid (MeSH) ; Amyloid Precursor Protein Secretases: metabolism (MeSH) ; Animals (MeSH) ; Antigens, CD: cerebrospinal fluid (MeSH) ; Antigens, CD: metabolism (MeSH) ; Aspartic Acid Endopeptidases: antagonists & inhibitors (MeSH) ; Aspartic Acid Endopeptidases: cerebrospinal fluid (MeSH) ; Aspartic Acid Endopeptidases: metabolism (MeSH) ; Astrocytes: metabolism (MeSH) ; Brain: cytology (MeSH) ; Brain: metabolism (MeSH) ; Cells, Cultured (MeSH) ; Cerebrospinal Fluid Proteins (MeSH) ; Chromatography, Liquid (MeSH) ; Gene Ontology (MeSH) ; Lipopolysaccharides: pharmacology (MeSH) ; Mice (MeSH) ; Mice, Inbred C57BL (MeSH) ; Microglia: metabolism (MeSH) ; Nerve Tissue Proteins: cerebrospinal fluid (MeSH) ; Nerve Tissue Proteins: metabolism (MeSH) ; Neurons: metabolism (MeSH) ; Oligodendroglia: metabolism (MeSH) ; Principal Component Analysis (MeSH) ; Proteome: metabolism (MeSH) ; Proteomics: methods (MeSH) ; Software (MeSH) ; Tandem Mass Spectrometry (MeSH) ; CSF ; BACE1 ; brain cells ; proteomics ; secretomics

Note: ISSN 1460-2075 not unique: **3 hits**.

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Contributing Institute(s):

1. Neuroproteomics (AG Lichtenthaler)
2. Molecular Neurodegeneration (AG Haass)
3. Juvenile Neurodegeneration (AG Tahirovic)
4. Molecular Neurobiology (AG Simons)
5. Library and Information Services (CRFS-LIS) (LIS)

Research Program(s):

1. 342 - Disease Mechanisms and Model Systems (POF3-342) (POF3-342)
2. 341 - Molecular Signaling (POF3-341) (POF3-341)

Appears in the scientific report 2020

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Database coverage:
; ; ; BIOSIS Previews ; Biological Abstracts ; Clarivate Analytics Master Journal List ; Current Contents - Life Sciences ; DEAL Wiley ; Ebsco Academic Search ; Essential Science Indicators ; IF >= 10 ; JCR ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection

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Linked articles:

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Dataset Müller, S. A. (First author)DZNE* ; Lichtenthaler, S. (Last author)DZNE*
Dataset: Quantitative secretome analysis using improved secretome-protein-enrichment-with-click-sugars method (iSPECS) establishes the cell type-resolved mouse brain glyco-secretome
PRoteomics IDEntifications Database (2020)2020   Fulltext BibTeX | EndNote: XML, Text | RIS

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Dataset Müller, S. A. (First author)DZNE* ; Lichtenthaler, S. (Last author)DZNE*
Dataset: Secretome Analysis of hippocampal and cortical murine neurons
PRoteomics IDEntifications Database (2020)2020   Fulltext BibTeX | EndNote: XML, Text | RIS

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