Journal Article

DZNE-2023-00328

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Microglial motility is modulated by neuronal activity and correlates with dendritic spine plasticity in the hippocampus of awake mice.

Nebeling, F. C. (First author)DZNE* ; Poll, S.DZNE* ; Justus, L. C.DZNE* ; Steffen, J.DZNE* ; Keppler, K.DZNE* ; Mittag, M.DZNE* ; Fuhrmann, M. (Last author)DZNE*

2023
eLife Sciences Publications Cambridge

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Please use a persistent id in citations: doi:10.7554/eLife.83176

Abstract: Microglia, the resident immune cells of the brain, play a complex role in health and disease. They actively survey the brain parenchyma by physically interacting with other cells and structurally shaping the brain. Yet, the mechanisms underlying microglial motility and significance for synapse stability, especially in the hippocampus during adulthood, remain widely unresolved. Here, we investigated the effect of neuronal activity on microglial motility and the implications for the formation and survival of dendritic spines on hippocampal CA1 neurons in vivo. We used repetitive two-photon in vivo imaging in the hippocampus of awake and anesthetized mice to simultaneously study the motility of microglia and their interaction with dendritic spines. We found that CA3 to CA1 input is sufficient to modulate microglial process motility. Simultaneously, more dendritic spines emerged in mice after awake compared to anesthetized imaging. Interestingly, the rate of microglial contacts with individual dendritic spines and dendrites was associated with the stability, removal, and emergence of dendritic spines. These results suggest that microglia might sense neuronal activity via neurotransmitter release and actively participate in synaptic rewiring of the hippocampal neural network during adulthood. Further, this study has profound relevance for hippocampal learning and memory processes.

Keyword(s): Mice (MeSH) ; Animals (MeSH) ; Microglia: physiology (MeSH) ; Dendritic Spines: physiology (MeSH) ; Wakefulness (MeSH) ; Hippocampus: physiology (MeSH) ; Neurons (MeSH) ; Neuronal Plasticity: physiology (MeSH) ; chemogenetics ; dendritic spines ; hippocampus ; microglia ; mouse ; neuroscience ; two-photon

Note: CC BY

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Contributing Institute(s):

1. Neuroimmunology and Imaging (AG Fuhrmann)
2. Light Microscopy Facility (LMF) (LMF)

Research Program(s):

1. 352 - Disease Mechanisms (POF4-352) (POF4-352)

Experiment(s):

1. Light Microscope Facility (CRFS-LMF) / Bonn

Appears in the scientific report 2023

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