Dataset

DZNE-2025-00814

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Dataset: Analysis of conformational changes induced by binding of allosteric nanobodies to a bacterial LRRK2 homologue by CX-MS

Gloeckner, C. J.DZNE*

2024
PRoteomics IDEntifications Database

Abstract: As part of an in-depth characterization of nanobodies directed against the Chlorobium tepidum protein CtRoco, a bacterial LRRK2 homologue, chemical crosslinking combined with mass spectrometry (CX-MS) was performed. Two allosteric nanobodies binding to two different epitopes in the LRR and the Roc domain of CtRoco, respectively were analysed using the CID-cleavable crosslinker disuccinimidyl sulfoxide (DSSO).

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Contributing Institute(s):

1. Functional Neuroproteomics and Translational Biomarkers in Neurodegenerative Diseases (AG Gloeckner)

Research Program(s):

1. 352 - Disease Mechanisms (POF4-352) (POF4-352)

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Linked articles:

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Journal Article Galicia, C. ; Guaitoli, G.DZNE* ; Fislage, M. ; Gloeckner, C. J.DZNE* ; Versées, W.
Structural insights into the GTP-driven monomerization and activation of a bacterial LRRK2 homolog using allosteric nanobodies.
eLife 13, RP94503 (2024) [10.7554/eLife.94503]2024   Files  Fulltext by Pubmed Central BibTeX | EndNote: XML, Text | RIS

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