Journal Article (Review Article)

DZNE-2020-06068

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Organoid technology for retinal repair.

Llonch, S. ; Carido, M.DZNE* ; Ader, M. (Corresponding author)

2018
Elsevier Amsterdam [u.a.]

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Please use a persistent id in citations: doi:10.1016/j.ydbio.2017.09.028

Abstract: A major cause for vision impairment and blindness in industrialized countries is the loss of the light-sensing retinal tissue in the eye. Photoreceptor damage is one of the main characteristics found in retinal degeneration diseases, such as Retinitis Pigmentosa or age-related macular degeneration. The lack of effective therapies to stop photoreceptor loss together with the absence of significant intrinsic regeneration in the human retina converts such degenerative diseases into permanent conditions that are currently irreversible. Cell replacement by means of photoreceptor transplantation has been proposed as a potential approach to tackle cell loss in the retina. Since the first attempt of photoreceptor transplantation in humans, about twenty years ago, several research groups have focused in the development and improvement of technologies necessary to bring cell transplantation for retinal degeneration diseases to reality. Progress in recent years in the generation of human tissue derived from pluripotent stem cells (PSCs) has significantly improved our tools to study human development and disease in the dish. Particularly the availability of 3D culture systems for the generation of PSC-derived organoids, including the human retina, has dramatically increased access to human material for basic and medical research. In this review, we focus on important milestones towards the generation of transplantable photoreceptor precursors from PSC-derived retinal organoids and discuss recent pre-clinical transplantation studies using organoid-derived photoreceptors in context to related in vivo work using primary photoreceptors as donor material. Additionally, we summarize remaining challenges for developing photoreceptor transplantation towards clinical application.

Keyword(s): Translational Research, Biomedical (MeSH) ; Animals (MeSH) ; Cellular Reprogramming Techniques (MeSH) ; Culture Media, Serum-Free: pharmacology (MeSH) ; Embryonic Stem Cells: cytology (MeSH) ; Embryonic Stem Cells: drug effects (MeSH) ; Humans (MeSH) ; Induced Pluripotent Stem Cells: transplantation (MeSH) ; Mice (MeSH) ; Morphogenesis (MeSH) ; Organoids: transplantation (MeSH) ; Photoreceptor Cells, Vertebrate: transplantation (MeSH) ; Pluripotent Stem Cells: transplantation (MeSH) ; Retina: cytology (MeSH) ; Retinal Degeneration: therapy (MeSH) ; Species Specificity (MeSH) ; Tissue Culture Techniques (MeSH) ; Translational Medical Research (MeSH) ; Culture Media, Serum-Free

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Contributing Institute(s):

1. Retinal Regeneration and Degeneration (AG Karl)

Research Program(s):

1. 342 - Disease Mechanisms and Model Systems (POF3-342) (POF3-342)

Appears in the scientific report 2018

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Database coverage:
; ; ; BIOSIS Previews ; Clarivate Analytics Master Journal List ; Current Contents - Life Sciences ; Ebsco Academic Search ; IF < 5 ; JCR ; Nationallizenz ; SCOPUS ; Web of Science Core Collection ; Zoological Record

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