Journal Article

DZNE-2020-06431

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Epigenome-wide DNA methylation profiling in Progressive Supranuclear Palsy reveals major changes at DLX1.

Weber, A. (Corresponding author) ; Schwarz, S. C.DZNE* ; Tost, J. ; Trümbach, D. ; Winter, P. ; Busato, F. ; Tacik, P. ; Windhorst, A. C. ; Fagny, M. ; Arzberger, T.DZNE* ; McLean, C. ; van Swieten, J. C. ; Schwarz, J. ; Vogt-Weisenhorn, D.DZNE* ; Wurst, W.DZNE* ; Adhikary, T. ; Dickson, D. W. ; Höglinger, G. U.DZNE* ; Müller, U.

2018
Nature Publishing Group UK [London]

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Please use a persistent id in citations: doi:10.1038/s41467-018-05325-y

Abstract: Genetic, epigenetic, and environmental factors contribute to the multifactorial disorder progressive supranuclear palsy (PSP). Here, we study epigenetic changes by genome-wide analysis of DNA from postmortem tissue of forebrains of patients and controls and detect significant (P < 0.05) methylation differences at 717 CpG sites in PSP vs. controls. Four-hundred fifty-one of these sites are associated with protein-coding genes. While differential methylation only affects a few sites in most genes, DLX1 is hypermethylated at multiple sites. Expression of an antisense transcript of DLX1, DLX1AS, is reduced in PSP brains. The amount of DLX1 protein is increased in gray matter of PSP forebrains. Pathway analysis suggests that DLX1 influences MAPT-encoded Tau protein. In a cell system, overexpression of DLX1 results in downregulation of MAPT while overexpression of DLX1AS causes upregulation of MAPT. Our observations suggest that altered DLX1 methylation and expression contribute to pathogenesis of PSP by influencing MAPT.

Keyword(s): Aged (MeSH) ; Aged, 80 and over (MeSH) ; DNA Methylation: genetics (MeSH) ; Epigenesis, Genetic: genetics (MeSH) ; Female (MeSH) ; Homeodomain Proteins: genetics (MeSH) ; Homeodomain Proteins: metabolism (MeSH) ; Humans (MeSH) ; Male (MeSH) ; Supranuclear Palsy, Progressive: genetics (MeSH) ; Supranuclear Palsy, Progressive: pathology (MeSH) ; Transcription Factors: genetics (MeSH) ; Transcription Factors: metabolism (MeSH) ; tau Proteins: genetics (MeSH) ; tau Proteins: metabolism (MeSH) ; Distal-less homeobox proteins ; Homeodomain Proteins ; MAPT protein, human ; Transcription Factors ; tau Proteins

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Contributing Institute(s):

1. Translational Neurodegeneration (AG Höglinger 1)
2. Clinical Neurodegeneration (AG Levin)
3. Ext HZM (Ext HZM)
4. Genome Engineering (AG Wurst)

Research Program(s):

1. 342 - Disease Mechanisms and Model Systems (POF3-342) (POF3-342)
2. 344 - Clinical and Health Care Research (POF3-344) (POF3-344)

Appears in the scientific report 2018

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