Journal Article

DZNE-2022-00936

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Vibration enhanced cell growth induced by surface acoustic waves as in vitro wound-healing model.

Brugger, M. S. ; Baumgartner, K. ; Mauritz, S. C. F. ; Gerlach, S. C. ; Röder, F. ; Schlosser, C.Extern* ; Fluhrer, R.DZNE* ; Wixforth, A. ; Westerhausen, C.

2020
National Acad. of Sciences Washington, DC

This record in other databases:    

Please use a persistent id in citations: doi:10.1073/pnas.2005203117

Abstract: We report on in vitro wound-healing and cell-growth studies under the influence of radio-frequency (rf) cell stimuli. These stimuli are supplied either by piezoactive surface acoustic waves (SAWs) or by microelectrode-generated electric fields, both at frequencies around 100 MHz. Employing live-cell imaging, we studied the time- and power-dependent healing of artificial wounds on a piezoelectric chip for different cell lines. If the cell stimulation is mediated by piezomechanical SAWs, we observe a pronounced, significant maximum of the cell-growth rate at a specific SAW amplitude, resulting in an increase of the wound-healing speed of up to 135 ± 85% as compared to an internal reference. In contrast, cells being stimulated only by electrical fields of the same magnitude as the ones exposed to SAWs exhibit no significant effect. In this study, we investigate this effect for different wavelengths, amplitude modulation of the applied electrical rf signal, and different wave modes. Furthermore, to obtain insight into the biological response to the stimulus, we also determined both the cell-proliferation rate and the cellular stress levels. While the proliferation rate is significantly increased for a wide power range, cell stress remains low and within the normal range. Our findings demonstrate that SAW-based vibrational cell stimulation bears the potential for an alternative method to conventional ultrasound treatment, overcoming some of its limitations.

Keyword(s): Acoustic Stimulation: adverse effects (MeSH) ; Acoustic Stimulation: instrumentation (MeSH) ; Acoustic Stimulation: methods (MeSH) ; Animals (MeSH) ; Cell Line (MeSH) ; Cell Line, Tumor (MeSH) ; Cell Movement: radiation effects (MeSH) ; Cell Proliferation: radiation effects (MeSH) ; Combined Modality Therapy: adverse effects (MeSH) ; Combined Modality Therapy: instrumentation (MeSH) ; Combined Modality Therapy: methods (MeSH) ; Dogs (MeSH) ; Electrodes (MeSH) ; Humans (MeSH) ; Madin Darby Canine Kidney Cells (MeSH) ; Oxidative Stress: drug effects (MeSH) ; Reactive Oxygen Species (MeSH) ; Sound: adverse effects (MeSH) ; Vibration: therapeutic use (MeSH) ; Wound Healing: radiation effects (MeSH) ; cell growth ; cell migration ; stimulation ; surface acoustic waves ; vibration ; Reactive Oxygen Species

---

Contributing Institute(s):

1. München common (München common)

Research Program(s):

1. 899 - ohne Topic (POF4-899) (POF4-899)

Appears in the scientific report 2020

---

Database coverage:
; BIOSIS Previews ; Biological Abstracts ; Clarivate Analytics Master Journal List ; Current Contents - Agriculture, Biology and Environmental Sciences ; Current Contents - Life Sciences ; Ebsco Academic Search ; Essential Science Indicators ; IF >= 10 ; JCR ; National-Konsortium ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection ; Zoological Record

---