Book/Journal Article

DZNE-2020-04656

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Genome Editing in Mice Using TALE Nucleases.

Wefers, B. (First author)DZNE* ; Brandl, C. ; Ortiz, O. ; Wurst, W.DZNE* ; Kühn, R.

2016
[Springer] [Heidelberg]
ISBN: 978-1-4939-2931-3 (print), 978-1-4939-2932-0 (electronic)

This record in other databases:    

Please use a persistent id in citations: doi:10.1007/978-1-4939-2932-0_17

Abstract: Gene engineering for generating targeted mouse mutants is a key technology for biomedical research. Using TALENs as sequence-specific nucleases to induce targeted double-strand breaks, the mouse genome can be directly modified in zygotes in a single step without the need for embryonic stem cells. By embryo microinjection of TALEN mRNAs and targeting vectors, knockout and knock-in alleles can be generated fast and efficiently. In this chapter we provide protocols for the application of TALENs in mouse zygotes.

Keyword(s): Animals (MeSH) ; Animals, Genetically Modified: genetics (MeSH) ; DNA Breaks, Double-Stranded (MeSH) ; Endonucleases: genetics (MeSH) ; Gene Knockout Techniques: methods (MeSH) ; Genome (MeSH) ; Mice (MeSH) ; Microinjections (MeSH) ; Mutation (MeSH) ; RNA Editing: genetics (MeSH) ; RNA, Messenger: genetics (MeSH) ; RNA, Messenger ; Endonucleases

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Contributing Institute(s):

1. Genome Engineering (AG Wurst)

Research Program(s):

1. 342 - Disease Mechanisms and Model Systems (POF3-342) (POF3-342)

Appears in the scientific report 2016

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Database coverage:
; NCBI Molecular Biology Database ; SCOPUS

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