Journal Article

DZNE-2023-00397

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Determining the Physico-Chemical Composition of Biomolecular Condensates from Spatially-Resolved NMR.

Pantoja Rivillas, C. (First author)DZNE* ; Ibáñez de Opakua, A.DZNE* ; Cima Omori, M. S.DZNE* ; Zweckstetter, M. (Last author)DZNE*

2023
Wiley-VCH Weinheim

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Please use a persistent id in citations: doi:10.1002/anie.202218078

Abstract: Liquid-Liquid phase separation has emerged as fundamental process underlying the formation of biomolecular condensates. Insights into the composition and structure of biomolecular condensates is, however, complicated by their molecular complexity and dynamics. Here, we introduce an improved spatially-resolved NMR experiment that enables quantitative analysis of the physico-chemical composition of multi-component biomolecular condensates in equilibrium and label-free. Application of spatially-resolved NMR to condensates formed by the Alzheimer's disease-associated protein Tau demonstrates decreased water content, exclusion of the molecular crowding agent dextran, presence of a specific chemical environment of the small molecule DSS, and ≈150-fold increased concentration of Tau inside the condensate. The results suggest that spatially-resolved NMR can have a major impact in understanding the composition and physical chemistry of biomolecular condensates.

Keyword(s): Humans (MeSH) ; 14-3-3 Proteins (MeSH) ; Alzheimer Disease (MeSH) ; Biomolecular Condensates (MeSH) ; Chemistry, Physical (MeSH) ; Magnetic Resonance Imaging (MeSH) ; Chemical Phenomena (MeSH) ; 14-3-3 Proteins ; Biomolecular condensates ; Multicomponent quantification ; Phase diagram ; Spatially-resolved NMR ; Tau protein

Note: CC BY-NC-ND

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Contributing Institute(s):

1. Structural Biology in Dementia (AG Zweckstetter)

Research Program(s):

1. 352 - Disease Mechanisms (POF4-352) (POF4-352)

Appears in the scientific report 2023

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Database coverage:
; ; ; Chemical Reactions ; Clarivate Analytics Master Journal List ; Current Contents - Life Sciences ; Current Contents - Physical, Chemical and Earth Sciences ; DEAL Wiley ; Ebsco Academic Search ; Essential Science Indicators ; IF >= 15 ; Index Chemicus ; JCR ; Nationallizenz ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection

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