Journal Article

DZNE-2024-01404

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Blood-based quantification of Aβ oligomers indicates impaired clearance from brain in ApoE ε4 positive subjects.

Blömeke, L. ; Rehn, F. ; Pils, M. ; Kraemer-Schulien, V. ; Cousin, A. ; Kutzsche, J. ; Bujnicki, T. ; Freiesleben, S. D.DZNE* ; Schneider, L.-S.DZNE* ; Preis, L.DZNE* ; Priller, J.DZNE* ; Spruth, E. J.DZNE* ; Altenstein, S.DZNE* ; Schneider, A.DZNE* ; Fliessbach, K.DZNE* ; Wiltfang, J.DZNE* ; Hansen, N. ; Rostamzadeh, A. ; Düzel, E.DZNE* ; Glanz, W.DZNE* ; Incesoy, E. I.DZNE* ; Buerger, K.DZNE* ; Janowitz, D.Extern* ; Ewers, M.DZNE* ; Perneczky, R.DZNE* ; Rauchmann, B. S.Extern* ; Teipel, S.DZNE* ; Kilimann, I.DZNE* ; Laske, C.DZNE* ; Munk, M. H.DZNE* ; Spottke, A.DZNE* ; Roy, N.DZNE* ; Heneka, M.Extern* ; Brosseron, F.DZNE* ; Wagner, M.DZNE* ; Roeske, S.DZNE* ; Ramirez, A.DZNE* ; Schmid, M.DZNE* ; Jessen, F.DZNE* ; Bannach, O. ; Peters, O.DZNE* ; Willbold, D.

2024
Springer Nature [London]

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Please use a persistent id in citations: doi:10.1038/s43856-024-00690-w

Abstract: Quantification of Amyloid beta (Aβ) oligomers in plasma enables early diagnosis of Alzheimer's Disease (AD) and improves our understanding of underlying pathologies. However, quantification necessitates an extremely sensitive and selective technology because of very low Aβ oligomer concentrations and possible interference from matrix components.In this report, we developed and validated a surface-based fluorescence distribution analysis (sFIDA) assay for quantification of Aβ oligomers in plasma.The blood-based sFIDA assay delivers a sensitivity of 1.8 fM, an inter- and intra-assay variation below 20% for oligomer calibration standards and no interference with matrix components. Quantification of Aβ oligomers in 359 plasma samples from the DELCODE cohort reveals lower oligomer concentrations in subjective cognitive decline and AD patients than healthy Control participants.Correlation analysis between CSF and plasma oligomer concentrations indicates an impaired clearance of Aβ oligomers that is dependent on the ApoE ε4 status.

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Contributing Institute(s):

1. Biomarker-Assisted Early Detection of Dementias (AG Peters)
2. Vascular Pathology (AG Dirnagl)
3. Interdisciplinary Dementia Research (AG Endres)
4. Clinical Neurophysiology and Memory (AG Düzel)
5. Translational Neuropsychiatry (AG Priller)
6. Translational Dementia Research (Bonn) (AG Schneider)
7. Patient Studies (Bonn) (Patient Studies (Bonn))
8. Molecular biomarkers for predictive diagnostics of neurodegenerative diseases (AG Wiltfang)
9. Clinical Research (Munich) (Clinical Research (Munich))
10. Vascular Cognitive Impairment & Post-Stroke Dementia (AG Dichgans)
11. Clinical Dementia Research (Rostock /Greifswald) (AG Teipel)
12. Parkinson Genetics (AG Gasser)
13. Clinical Research Platform (CRP) (AG Spottke)
14. Clinical Research Platform (CRP) (Clinical Research Platform (CRP))
15. Neuropsychology (AG Wagner)
16. Clinical Alzheimer’s Disease Research (AG Jessen)
17. Mathematics, statistics and informatics methods for support of population studies and clinical research (AG Schmid Bonn)
18. Neuroinflammation, Biomarker (AG Heneka)

Research Program(s):

1. 353 - Clinical and Health Care Research (POF4-353) (POF4-353)

Appears in the scientific report 2024

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Database coverage:
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