Contribution to a book

DZNE-2026-00151

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Laser-Capture Microdissection Followed by ATAC-Seq.

Hartmann, C. L. (First author)DZNE* ; Schultze, J. L.DZNE* ; Beyer, M. D.DZNE* ; Carraro, C. (Last author)DZNE*

2026
Springer US New York, NY
ISBN: 978-1-0716-5153-7 (print), 978-1-0716-5154-4 (electronic)

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Please use a persistent id in citations: doi:10.1007/978-1-0716-5154-4_12

Abstract: Laser-capture microdissection (LCM) enables the collection of regions of interest from target tissues, making it compatible with a number of spatially resolved downstream omics applications. Here, we provide a detailed protocol for LCM coupled to ATAC-seq (LCM-ATAC-seq) for the spatial analysis of chromatin landscape based on the method published by Carraro et al. (Cell Rep Methods 3:100598, 2023). LCM-ATAC-seq allows the efficient capture down to single nuclei and can be combined with the morphological staining of target cell populations to characterize chromatin states in fresh-frozen tissue samples.

Keyword(s): Laser Capture Microdissection: methods (MeSH) ; Chromatin: genetics (MeSH) ; Chromatin Immunoprecipitation Sequencing: methods (MeSH) ; Humans (MeSH) ; High-Throughput Nucleotide Sequencing: methods (MeSH) ; ATAC-seq ; Chromatin accessibility ; Laser-capture microdissection ; Morphological staining ; Spatial omics ; Chromatin

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Contributing Institute(s):

1. Clinical Single Cell Omics (CSCO) / Systems Medicine (AG Schultze)
2. Immunogenomics and Neurodegeneration (AG Beyer)
3. Platform for Single Cell Genomics and Epigenomics (PRECISE)

Research Program(s):

1. 354 - Disease Prevention and Healthy Aging (POF4-354) (POF4-354)
2. 351 - Brain Function (POF4-351) (POF4-351)
3. 352 - Disease Mechanisms (POF4-352) (POF4-352)

Experiment(s):

1. Platform for Single Cell Genomics and Epigenomics at DZNE University of Bonn

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Database coverage:
; SCOPUS

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