Spatiotemporal Mapping of Epileptiform Dynamics in the Hippocampal Circuit via High-Density Biosensors.

Emery, Brett Addison; Hu, Xin; Amin, Hayder; Khanzada, Shahrukh

doi:10.1109/EMBC58623.2025.11253841

Contribution to a conference proceedings/Contribution to a book

DZNE-2026-00342

Spatiotemporal Mapping of Epileptiform Dynamics in the Hippocampal Circuit via High-Density Biosensors.

Emery, B. A. (First author)DZNE* ; Khanzada, S.DZNE* ; Hu, X.DZNE* ; Amin, H. (Last author)DZNE*

2025
IEEE

2025 47th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC) : [Proceedings] - IEEE, 2025. - ISBN 979-8-3315-8618-8 - doi:10.1109/EMBC58623.2025.11253841
47th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC, Copenhagen, Denmark, 14 Jul 2025 - 18 Jul 2025 IEEE 1-4 (2025) [10.1109/EMBC58623.2025.11253841]

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Abstract: 70 million people suffer from epilepsy worldwide with temporal lobe epilepsy (TLE) being the most common focal epilepsy in adults. The hippocampus, a region associated with TLE, is highly susceptible to pathological synchronization and epileptogenesis due to its functional and anatomical organization. Despite progress, understanding the distinct contributions of hippocampal subregions to multiplexed activation and hyperactivity remains challenging due to the interplay of receptor dynamics, ion channel regulation, and network synchronization. Here, a large-scale ex-vivo electrophysiological platform was implemented using a high-density CMOS-based biosensor (HD-MEA) to probe the spatiotemporal dynamics of 4AP and ATPA, benchmark compounds for inducing hyperactivity, within the hippocampal circuit. This approach demonstrates the use of large-scale biosensors to address these challenges by enabling high-resolution spatiotemporal mapping of circuit activity, demonstrating their potential to uncover ion channel and receptor-specific spatiotemporal signatures of hyperactivity.

Keyword(s): Biosensing Techniques: methods (MeSH) ; Biosensing Techniques: instrumentation (MeSH) ; Hippocampus: physiopathology (MeSH) ; Animals (MeSH) ; Epilepsy: physiopathology (MeSH) ; Epilepsy, Temporal Lobe: physiopathology (MeSH) ; Spatio-Temporal Analysis (MeSH) ; Humans (MeSH)