Book/Journal Article

DZNE-2020-05038

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png In vivo imaging of mitochondria in intact zebrafish larvae.

Paquet, D. (First author)DZNE* ; Plucińska, G. ; Misgeld, T. (Last author)DZNE*

2014
Elsevier New York, NY [u.a.]

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Please use a persistent id in citations: doi:10.1016/B978-0-12-801415-8.00009-6

Abstract: Visualizing neuronal mitochondria in a living, intact mammalian organism is a challenge that can be overcome in zebrafish larvae, which are highly accessible for optical imaging and genetic manipulation. Here, we detail an approach to visualize neuronal mitochondria in sensory Rohon-Beard axons, which allows quantitatively measuring mitochondrial shape, dynamics, and transport in vivo. This provides a useful assay for basic studies exploring the behavior of neuronal mitochondria in their natural habitat, for revealing the influence that disease-related alterations have on this behavior and for testing pharmacological compounds and genetic manipulations that might ameliorate disease-related mitochondrial phenotypes in neurons.

Keyword(s): Animals (MeSH) ; Animals, Genetically Modified (MeSH) ; Axonal Transport (MeSH) ; Axons (MeSH) ; Bacterial Proteins: genetics (MeSH) ; Bacterial Proteins: metabolism (MeSH) ; Electronic Data Processing (MeSH) ; Embryo, Nonmammalian: cytology (MeSH) ; Image Processing, Computer-Assisted (MeSH) ; Larva: cytology (MeSH) ; Luminescent Proteins: genetics (MeSH) ; Luminescent Proteins: metabolism (MeSH) ; Microscopy, Fluorescence: instrumentation (MeSH) ; Microscopy, Fluorescence: methods (MeSH) ; Mitochondria: physiology (MeSH) ; Sensory Receptor Cells: cytology (MeSH) ; Zebrafish: embryology (MeSH) ; Zebrafish: genetics (MeSH) ; Bacterial Proteins ; Luminescent Proteins ; yellow fluorescent protein, Bacteria

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Contributing Institute(s):

1. Fish Core Unit (AG Schmid)
2. Neuronal Cell Biology (AG Misgeld)

Research Program(s):

1. 341 - Molecular Signaling (POF3-341) (POF3-341)

Appears in the scientific report 2014

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Database coverage:
; BIOSIS Previews ; BIOSIS Reviews Reports And Meetings ; Clarivate Analytics Master Journal List ; IF < 5 ; JCR ; NCBI Molecular Biology Database ; SCOPUS ; Science Citation Index ; Science Citation Index Expanded ; Web of Science Core Collection

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