Journal Article

DZNE-2022-00002

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png The anterior paired lateral neuron normalizes odour-evoked activity in the Drosophila mushroom body calyx.

Prisco, L. (First author)DZNE* ; Deimel, S. H.Extern* ; Yeliseyeva, H.DZNE* ; Fiala, A.Extern* ; Tavosanis, G. (Last author)DZNE*

2021
eLife Sciences Publications Cambridge

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Please use a persistent id in citations: doi:10.7554/eLife.74172

Abstract: To identify and memorize discrete but similar environmental inputs, the brain needs to distinguish between subtle differences of activity patterns in defined neuronal populations. The Kenyon cells (KCs) of the Drosophila adult mushroom body (MB) respond sparsely to complex olfactory input, a property that is thought to support stimuli discrimination in the MB. To understand how this property emerges, we investigated the role of the inhibitory anterior paired lateral (APL) neuron in the input circuit of the MB, the calyx. Within the calyx, presynaptic boutons of projection neurons (PNs) form large synaptic microglomeruli (MGs) with dendrites of postsynaptic KCs. Combining electron microscopy (EM) data analysis and in vivo calcium imaging, we show that APL, via inhibitory and reciprocal synapses targeting both PN boutons and KC dendrites, normalizes odour-evoked representations in MGs of the calyx. APL response scales with the PN input strength and is regionalized around PN input distribution. Our data indicate that the formation of a sparse code by the KCs requires APL-driven normalization of their MG postsynaptic responses. This work provides experimental insights on how inhibition shapes sensory information representation in a higher brain centre, thereby supporting stimuli discrimination and allowing for efficient associative memory formation.

Keyword(s): Animals (MeSH) ; Calcium: analysis (MeSH) ; Drosophila melanogaster: physiology (MeSH) ; Female (MeSH) ; Male (MeSH) ; Microscopy, Confocal (MeSH) ; Microscopy, Electron (MeSH) ; Mushroom Bodies: physiology (MeSH) ; Mushroom Bodies: ultrastructure (MeSH) ; Neurons: physiology (MeSH) ; Neurons: ultrastructure (MeSH) ; Presynaptic Terminals (MeSH) ; Smell: physiology (MeSH) ; APL ; D. melanogaster ; inhibition ; microglomerulus ; mushroom body ; neuroscience ; pattern separation ; sparse coding ; Calcium

Note: Grants relevant to the publication: Forschungsgruppe 2705 , Entschlüsselung eines Gehirn-Schaltkreises: Struktur, Plastizität und Verhaltensfunktion des Pilzkörpers von Drosophila

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Contributing Institute(s):

1. Dynamics of neuronal circuits (AG Tavosanis)

Research Program(s):

1. 351 - Brain Function (POF4-351) (POF4-351)

Appears in the scientific report 2021

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Database coverage:
; ; ; ; Article Processing Charges ; BIOSIS Previews ; Biological Abstracts ; Clarivate Analytics Master Journal List ; DOAJ Seal ; Ebsco Academic Search ; Essential Science Indicators ; Fees ; IF >= 5 ; JCR ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection ; Zoological Record

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Linked articles:

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Software Tavosanis, G.DZNE* ; Prisco, L.DZNE*
Software: APL synapses distribution on PN and KC meshes, primary data, calcium imaging macros and python scripts from Prisco et al. (v1)
Zenodo (2022) [10.5281/ZENODO.5904586]2022 BibTeX | EndNote: XML, Text | RIS

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Dataset Tavosanis, G.Extern* ; Prisco, L. (Last author)DZNE*
Dataset: APL synapses distribution on PN and KC meshes, primary data, calcium imaging macros and python scripts from Prisco et al.
Dryad (2021) [10.5061/dryad.bk3j9kdd1]2021 BibTeX | EndNote: XML, Text | RIS

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